In the current investigation, we have reported on the preparation of Na-Mordenite (MOR) modified by tryptophan (MOR-NH2) nanocomposite was synthesized and characterized using FT-IR, XRD, SEM, XPS, and BET that represented that the MOR-NH2 has high surface area 288 m2/g and pore volume 0.38 cm3/g. This composite represented high efficiency in removal of food dye Azorubine (E122) was 1043 mg/g. Study all the factors that affected on the adsorption such as pH, dose, salinity, E122 dye concentration as well as study the adsorption isotherm models that represented that was fitted to Langmuir. Moreover, study the effect of time according to it the adsorption process was fitted to Pseudo-second-order, and the effect of temperature that approved that the reaction was endothermic, spontaneous, and chemisorption process. pHzpc was determined to be 6.2 that mean that favorable to adsorb the anionic dyes such as E122 dye to <6.2. The adsorption process was optimized using Box-Behnken Design (BBD). The MOR-NH2 adsorbent demonstrated a simple and effective model for water purification and the management of industrial wastewater specially the regeneration efficiency of the adsorbent that led to use this adsorbent for five cycles. The MOR-NH2 nanocomposite was tested and proven to effectively inhibit the growth of Escherichia coli ATCC® 25922™ and Staphylococcus aureus ATCC® 25923™ at low concentrations. The impressive antibacterial capabilities and dye removal of the ZMOR-NH2 nanocomposite make it an excellent choice for antibacterial solutions! To the best of our knowledge, this work is the first to report the usage of MOR-NH2 adsorbents for the removal of E122 dye in wastewater samples. The effects of adsorption process the effects of adsorption process. The mechanism of interaction between MOR-NH2 and E122 dye was determining as it could be through Hydrogen bonding, pore filling, or through π- π interaction. This research offers a promising solution for purifying water sources that are contaminated with a variety of chemicals, microorganisms, and other contaminants. With the potential for future breakthroughs in purification methods, this study is envisioned to lead the way for new nanocomposite materials which could be used to remove an array of pollutants -from hazardous chemicals to viruses and bacteria.