PREVALENCE OF INFECTION AND GENETIC DIVERSITY OF PORCINE CIRCOVIRUS TYPE 2 (PCV2) IN WILD BOAR (SUS SCROFA) IN POLAND

Porcine circovirus type 2 (PCV2) is a widespread, important pathogen of domestic swine and the causative agent of postweaning multisystemic wasting syndrome and other diseases and conditions referred to as “porcine circovirus diseases.” Specific antibodies and DNA to PCV2 have also been detected in European wild boars and North American feral pigs. We collected 312 tonsil samples from wild boars shot in 13 of 16 districts of Poland, and tested them for PCV2 DNA using a real-time PCR. We detected PCV2 DNA in 75.6% of tested tonsils, and in particular, in 60% of samples from the 2006–07 season, and 91% from 2007–08. The phylogenetic analysis that included 12 PCV2 sequences from wild boars revealed that they belonged to two genetic clusters, PCV2b and PCV2a. We present data on prevalence of PCV2 in Polish wild boars and for the first time report the PCV2a genotype in Poland.

[1]  M. Spînu,et al.  Prevalence of porcine circoviruses in Transylvanian wild boars, detected by real-time PCR--short communication. , 2010, Acta veterinaria Hungarica.

[2]  Changming Liu,et al.  Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China , 2010, Virology Journal.

[3]  G. Reiner,et al.  Qualitative and quantitative distribution of PCV2 in wild boars and domestic pigs in Germany. , 2010, Veterinary microbiology.

[4]  A. Bastos,et al.  Role of Wild Suids in the Epidemiology of African Swine Fever , 2009, EcoHealth.

[5]  E. Holmes,et al.  Insights into the Evolutionary History of an Emerging Livestock Pathogen: Porcine Circovirus 2 , 2009, Journal of Virology.

[6]  G. Reiner,et al.  Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection in wild boars. , 2009, Veterinary microbiology.

[7]  S. Barocci,et al.  Detection of porcine circovirus type 2 (PCV2) from wild boars in central Italy , 2009, European Journal of Wildlife Research.

[8]  N. Knowles,et al.  DETECTION AND GENETIC CHARACTERIZATION OF PORCINE CIRCOVIRUS 2 ISOLATES FROM THE FIRST CASES OF POSTWEANING MULTISYSTEMIC AND WASTING SYNDROME IN WILD BOARS IN GREECE , 2008, Journal of wildlife diseases.

[9]  E. Bártová,et al.  Antibodies to Selected Viral Disease Agents in Wild Boars from the Czech Republic , 2008, Journal of wildlife diseases.

[10]  L. Larsen,et al.  Genomic analysis of PCV2 isolates from Danish archives and a current PMWS case-control study supports a shift in genotypes with time. , 2008, Veterinary microbiology.

[11]  J. Segalés,et al.  A review of viral diseases of the European wild boar: Effects of population dynamics and reservoir rôle , 2007, The Veterinary Journal.

[12]  À. Olvera,et al.  First description of postweaning multisystemic wasting syndrome (PMWS) in wild boar (Sus scrofa) in Croatia and phylogenetic analysis of partial PCV2 sequences. , 2007, Acta veterinaria Hungarica.

[13]  D. Driemeier,et al.  Postweaning multisystemic wasting syndrome in farmed wild boars (Sus scrofa) in Rio Grande do Sul , 2006 .

[14]  J. Segalés,et al.  Seroprevalence of six reproductive pathogens in European wild boar (Sus scrofa) from Spain: the effect on wild boar female reproductive performance. , 2006, Theriogenology.

[15]  I. Kiss,et al.  Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars , 2006, Archives of Virology.

[16]  G. Reiner,et al.  Comparative genetic characterization of Porcine Circovirus type 2 samples from German wild boar populations. , 2005, Veterinary microbiology.

[17]  C. Gortázar,et al.  Serosurvey of Aujeszky’s disease virus infection in European wild boar in Spain , 2005, Veterinary Record.

[18]  P. Hostnik,et al.  Phylogenetic analysis of type 2 porcine circoviruses identified in wild boar in Slovenia , 2004, Veterinary Record.

[19]  J. Segalés,et al.  Identification of postweaning multisystemic wasting syndrome in European wild boar (Sus scrofa) , 2004, Veterinary Record.

[20]  J. Segalés,et al.  Epidemiological study on porcine circovirus type 2 (PCV2) infection in the European wild boar (Sus scrofa). , 2004, Veterinary research.

[21]  D. Paton,et al.  Classical swine fever--an update. , 2003, Research in veterinary science.

[22]  B. Meehan,et al.  Porcine Circovirus 2–Associated Disease in Eurasian Wild Boar , 2003, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[23]  S. Sorden Update on porcine circovirus and postweaning multisystemic wasting syndrome (PMWS) , 2000 .

[24]  S. Dea,et al.  Multiplex PCR for Detection and Typing of Porcine Circoviruses , 1999, Journal of Clinical Microbiology.

[25]  Lihua Lin,et al.  Nucleotide Sequence of Porcine Circovirus Associated with Postweaning Multisystemic Wasting Syndrome in Pigs , 1998, Journal of Virology.

[26]  A. Bøtner,et al.  Novel porcine circoviruses from pigs with wasting disease syndromes. , 1998, The Veterinary record.