Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis.

Veritable identification and differentiation of avian species is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. Therefore, this study involved the application of molecular approach to identify some avian species i.e. Chicken (Gallus gallus), Muskovy duck (Cairina moschata), Japanese quail (Coturnix japonica), Laughing dove (Streptopelia senegalensis), and Rock pigeon (Columba livia). Genomic DNA was extracted from blood samples and partial sequence of the mitochondrial cytochrome b gene (358 bp) was amplified and sequenced using universal primers. Sequences alignment and phylogenetic analyses were performed by CLC main workbench program. The obtained five sequences were deposited in GenBank and compared with those previously registered in GenBank. The similarity percentage was 88.60% between Gallus gallus and Coturnix japonica and 80.46% between Gallus gallus and Columba livia. The percentage of identity between the studied species and GenBank species ranged from 77.20% (Columba oenas and Anas platyrhynchos) to 100% (Gallus gallus and Gallus sonneratii, Coturnix coturnix and Coturnix japonica, Meleagris gallopavo and Columba livia). Amplification of the partial sequence of mitochondrial cytochrome b gene proved to be practical for identification of an avian species unambiguously.

[1]  E. Michalska,et al.  Application of chicken microsatellite markers to molecular monitoring of the experimental population of Japanese quail (Coturnix japonica). , 2013 .

[2]  B. Shapiro,et al.  Nuclear DNA from the extinct Passenger Pigeon (Ectopistes migratorius) confirms a single origin of New World pigeons. , 2012, Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft.

[3]  E. Garcia-Vazquez,et al.  Beyond Biodiversity: Fish Metagenomes , 2011, PloS one.

[4]  A. Awan,et al.  DNA fingerprinting of Pakistani buffalo breeds (Nili-Ravi, Kundi) using microsatellite and cytochrome b gene markers , 2011, Molecular Biology Reports.

[5]  João Carneiro,et al.  Identification of species with DNA-based technology: current progress and challenges. , 2008, Recent patents on DNA & gene sequences.

[6]  T. García,et al.  An indirect ELISA and a PCR technique for the detection of Grouper ( Epinephelus marginatus ) mislabeling , 2008, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[7]  E. Miyauchi,et al.  PCR Method of Detecting Pork in Foods for Verifying Allergen Labeling and for Identifying Hidden Pork Ingredients in Processed Foods , 2007, Bioscience, biotechnology, and biochemistry.

[8]  M. Saini,et al.  Characterisation of peacock (Pavo cristatus) mitochondrial 12S rRNA sequence and its use in differentiation from closely related poultry species , 2007, British poultry science.

[9]  T. García,et al.  Analysis of mitochondrial DNA for authentication of meats from chamois (Rupicapra rupicapra), pyrenean ibex (Capra pyrenaica), and mouflon (Ovis ammon) by polymerase chain reaction-restriction fragment length polymorphism. , 2007, Journal of AOAC International.

[10]  A. Vignal,et al.  Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence , 2006, BMC Genomics.

[11]  A. Wilson,et al.  Congruency of phylogenies derived from different proteins , 1976, Journal of Molecular Evolution.

[12]  G. Zięba,et al.  Taxonomic position of Bison bison [Linnaeus 1758] and Bison bonasus [Linnaeus 1758] as determined by means of cytb gene sequence , 2004 .

[13]  S Sasazaki,et al.  Development of breed identification markers derived from AFLP in beef cattle. , 2004, Meat science.

[14]  A. Vignal,et al.  A first-generation microsatellite linkage map of the Japanese quail. , 2004, Animal Genetics.

[15]  Mark R. Wilson,et al.  Forensics and mitochondrial DNA: applications, debates, and foundations. , 2003, Annual review of genomics and human genetics (Print).

[16]  A. Rokas,et al.  Animal mitochondrial DNA recombination revisited , 2003 .

[17]  R. Osta,et al.  Random amplified polymorphic DNA fingerprints for identification of species in poultry pâté. , 2001, Poultry science.

[18]  Y. Matsuda,et al.  Chicken microsatellite primers are not efficient markers for Japanese quail. , 2001, Animal genetics.

[19]  M. Steinlechner,et al.  Species identification by means of the cytochrome b gene , 2000, International Journal of Legal Medicine.

[20]  R. Pérez-Martín,et al.  Use of restriction fragment length polymorphism to distinguish between salmon species. , 2000, Journal of agricultural and food chemistry.

[21]  T. Ozawa,et al.  Phylogenetic relationships among european red deer, wapiti, and sika deer inferred from mitochondrial DNA sequences. , 2000, Molecular phylogenetics and evolution.

[22]  S. W. Pang,et al.  Japanese quail microsatellite loci amplified with chicken-specific primers. , 1999, Animal genetics.

[23]  V. Loeschcke,et al.  Conservation Genetics , 2019, Handbook of Statistical Genomics.

[24]  A. Meyer,et al.  Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. , 1989, Proceedings of the National Academy of Sciences of the United States of America.