Nanosecond pulsed electric field stimulation of reactive oxygen species in human pancreatic cancer cells is Ca(2+)-dependent.

The cellular response to 100 ns pulsed electric fields (nsPEF) exposure includes the formation of transient nanopores in the plasma membrane and organelle membranes, an immediate increase in intracellular Ca(2+), an increase in reactive oxygen species (ROS), DNA fragmentation and caspase activation. 100 ns, 30 kV/cm nsPEF stimulates an increase in ROS proportional to the pulse number. This increase is inhibited by the anti-oxidant, Trolox, as well as the presence of Ca(2+) chelators in the intracellular and extracellular media. This suggests that the nsPEF-triggered Ca(2+) increase is required for ROS generation.

[1]  Y. Higuchi.  Glutathione depletion‐induced chromosomal DNA fragmentation associated with apoptosis and necrosis , 2004, Journal of cellular and molecular medicine.

[2]  J Teissié,et al.  The generation of reactive-oxygen species associated with long-lasting pulse-induced electropermeabilisation of mammalian cells is based on a non-destructive alteration of the plasma membrane. , 1999, Biochimica et biophysica acta.

[3]  U. Pliquett,et al.  Nanosecond pulsed electric fields cause melanomas to self-destruct , 2006, Conference Record of the 2006 Twenty-Seventh International Power Modulator Symposium.

[4]  Laura Marcu,et al.  In vitro and in vivo evaluation and a case report of intense nanosecond pulsed electric field as a local therapy for human malignancies , 2007, International journal of cancer.

[5]  J. Teissié,et al.  Generation of reactive-oxygen species induced by electropermeabilization of Chinese hamster ovary cells and their consequence on cell viability. , 1994, European journal of biochemistry.

[6]  Andrei G. Pakhomov,et al.  Analysis of Plasma Membrane Integrity by Fluorescent Detection of Tl+ Uptake , 2010, The Journal of Membrane Biology.

[7]  A. T. Esser,et al.  A brief overview of electroporation pulse strength-duration space: a region where additional intracellular effects are expected. , 2012, Bioelectrochemistry.

[8]  S. Roy,et al.  Bid-induced mitochondrial membrane permeabilization waves propagated by local reactive oxygen species (ROS) signaling , 2012, Proceedings of the National Academy of Sciences.

[9]  Shu Xiao,et al.  Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses. , 2011, Biochimica et biophysica acta.

[10]  Stephen J. Beebe,et al.  An apoptosis targeted stimulus with nanosecond pulsed electric fields (nsPEFs) in E4 squamous cell carcinoma , 2011, Apoptosis.

[11]  K. Schoenbach,et al.  Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition , 2001, PPPS-2001 Pulsed Power Plasma Science 2001. 28th IEEE International Conference on Plasma Science and 13th IEEE International Pulsed Power Conference. Digest of Papers (Cat. No.01CH37251).

[12]  K. Schoenbach,et al.  Differential effects in cells exposed to ultra-short, high intensity electric fields: cell survival, DNA damage, and cell cycle analysis. , 2003, Mutation research.

[13]  Richard Nuccitelli,et al.  Optimized nanosecond pulsed electric field therapy can cause murine malignant melanomas to self‐destruct with a single treatment , 2010, International journal of cancer.

[14]  Laura Marcu,et al.  Calcium bursts induced by nanosecond electric pulses. , 2003, Biochemical and biophysical research communications.

[15]  Juergen F. Kolb,et al.  A new pulsed electric field therapy for melanoma disrupts the tumor's blood supply and causes complete remission without recurrence , 2009, International journal of cancer.

[16]  Karl H. Schoenbach,et al.  Transient Features in Nanosecond Pulsed Electric Fields Differentially Modulate Mitochondria and Viability , 2012, PloS one.

[17]  Richard Nuccitelli,et al.  Non‐thermal Nanoelectroablation of UV‐induced Murine Melanomas Stimulates an Immune Response , 2012, Pigment cell & melanoma research.

[18]  K. Schoenbach,et al.  Nanosecond pulsed electric fields stimulate apoptosis without release of pro-apoptotic factors from mitochondria in B16f10 melanoma. , 2010, Archives of biochemistry and biophysics.

[19]  Karl H. Schoenbach,et al.  Stimulation of Capacitative Calcium Entry in HL-60 Cells by Nanosecond Pulsed Electric Fields* , 2004, Journal of Biological Chemistry.

[20]  Shu-sen Zheng,et al.  Tumor ablation with nanosecond pulsed electric fields. , 2012, Hepatobiliary & pancreatic diseases international : HBPD INT.

[21]  T. Takayama,et al.  Fas-Mediated Apoptosome Formation Is Dependent on Reactive Oxygen Species Derived from Mitochondrial Permeability Transition in Jurkat Cells1 , 2004, The Journal of Immunology.

[22]  Andrei G. Pakhomov,et al.  Electric Field Exposure Triggers and Guides Formation of Pseudopod-Like Blebs in U937 Monocytes , 2012, The Journal of Membrane Biology.

[23]  O. Pakhomova,et al.  Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media. , 2012, Archives of biochemistry and biophysics.

[24]  Richard Nuccitelli,et al.  Nanoelectroablation therapy for murine basal cell carcinoma. , 2012, Biochemical and biophysical research communications.

[25]  S. Beebe,et al.  Nanosecond pulsed electric fields (nsPEFs) activate intrinsic caspase-dependent and caspase-independent cell death in Jurkat cells. , 2012, Biochemical and biophysical research communications.

[26]  Karl H. Schoenbach,et al.  Nanosecond pulsed electric field ablation of hepatocellular carcinoma , 2011, 2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[27]  K. Schoenbach,et al.  Intracellular effect of ultrashort electrical pulses , 2001, Bioelectromagnetics.

[28]  Juergen F Kolb,et al.  Long‐lasting plasma membrane permeabilization in mammalian cells by nanosecond pulsed electric field (nsPEF) , 2007, Bioelectromagnetics.

[29]  Damijan Miklavčič,et al.  Nanosecond electric pulses cause mitochondrial membrane permeabilization in Jurkat cells , 2012, Bioelectromagnetics.

[30]  C. Dupuy,et al.  The emerging role of ROS-generating NADPH oxidase NOX4 in DNA-damage responses. , 2012, Mutation research.

[31]  J. Teissié,et al.  Electropermeabilization of intact maize cells induces an oxidative stress. , 1996, European journal of biochemistry.