Inhibition of Clostridium botulinum Type E Toxin Formation by Potassium Chloride and Sodium Chloride in Hot-Process (Smoked) Whitefish ( Coregonus clupeaformis ).

Whitefish steaks were brined in NaCl, KCl, or equimolar NaCl:KCl to contain similar chloride ion concentration and inoculated intramuscularly with 10 or 100 spores of Clostridium botulinum type E per gram of fish. Steaks were then heated in a simulated (i.e., without smoke) hot-smoke process to internal temperatures of 62.8° to 76.7°C (145°-170°F) for the final 30 min of a 2- to 3-h process, packaged under vacuum in oxygen-impermeable film, and stored at abuse temperature of 25°C. During 7 d of storage, toxin production was inhibited in steaks containing more than 0.66 ionic strength NaCl, 0.64 KCl, or 0.71 equimolar NaCl:KCl. The results indicate that it is feasible to substitute KCl for NaCl in hot-process smoked fish for inhibition of outgrowth and toxin production by Clostridium botulinum type E.

[1]  J. T. Duff,et al.  ACTIVATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN BY TRYPSIN , 1956, Journal of bacteriology.

[2]  D. Kautter.  Clostridium Botulinum Type E in Smoked Fish , 1964 .

[3]  A. Rothstein,et al.  Discrimination between Alkali Metal Cations by Yeast II. Cation interactions in transport , 1967 .

[4]  F. Harold,et al.  Inhibition of potassium transport by sodium in a mutant of Streptococcus faecalis. , 1967, Biochemistry.

[5]  L. N. Christiansen,et al.  Survival and outgrowth of Clostridium botulinum type E spores in smoked fish. , 1968, Applied microbiology.

[6]  A. Emodi,et al.  Low Temperature Growth of Type E Clostridium botulinum Spores. 2. Effects of Solutes and Incubation Temperature , 1969 .

[7]  R. Mandigo,et al.  EFFECT OF REDUCTION AND PARTIAL REPLACEMENT OF SODIUM ON BOLOGNA CHARACTERISTICS AND ACCEPTABILITY , 1980 .

[8]  W. Horwitz.  Official Methods of Analysis , 1980 .

[9]  E. H. Marth,et al.  Behavior of Staphylococcus aureus in Cheddar Cheese Made with Sodium Chloride or a Mixture of Sodium Chloride and Potassium Chloride. , 1982, Journal of food protection.

[10]  L. Hand,et al.  Effects of Complete or Partial Replacement of Sodium Chloride on Processing and Sensory Properties of Hams , 1982 .

[11]  L. Hand,et al.  Effects of Chloride Salts on Physical, Chemical and Sensory Properties of Frankfurters , 1982 .

[12]  R. C. Lindsay,et al.  Effect of Sodium/Potassium (1:1) Chloride and Low Sodium Chloride Concentrations on Quality of Cheddar Cheese , 1982 .

[13]  M. Eklund,et al.  Inhibition of Clostridium botulinum Types A and E Toxin Production by Liquid Smoke and NaCl in Hot-Process Smoke-Flavored Fish. , 1982, Journal of food protection.

[14]  R. N. Terrell.  Reducing the sodium content of processed meats , 1983 .

[15]  C. Vanderzant,et al.  Effect of Addition of Chloride Salts on Rancidity of Ground Pork Inoculated with a Moraxella or a Lactobacillus Species , 1983 .

[16]  C. Vanderzant,et al.  Effects of Reduction or Replacement of Sodium Chloride on Growth of Micrococcus, Moraxella and Lactobacillus Inoculated Ground Pork , 1983 .

[17]  J. T. Keeton.  Effects of Potassium Chloride on Properties of Country-Style Hams , 1984 .

[18]  J. Dwyer,et al.  Salt substitutes and medicinal potassium sources: risks and benefits. , 1985, Journal of the American Dietetic Association.