Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon

To conquer disease problem in shrimp industries, probiotic biocontrol is a well known remedy now. The antagonistic ability of separated isolates from different parts of juvenile Penaeus monodon were screened against shrimp Vibrio pathogens; Vibrio parahaemolyticus and Vibrio alginolyticus. The most antagonistic effect was observed for an isolate that was primarily identified as Shewanella algae using conventional methods followed by Biolog microlog software. Since production of antagonistic agents rely on cultural conditions, antagonistic ability of candidate probioic against the mentioned Vibrios was assessed using Response Surface Methodology, with central composite design in which four independents variables were assumed: temperature (10 - 50°C), pH (6 -10), NaCl concentration (0 - 50%) and time (12 – 60 h). The coefficients of multiple determinations (R2), for the responses of antagonistic effect of S. algae against V. parahaemolyticus and V. alginolyticus values were 0.807 and 0.805, respectively. Concentration of the NaCl exhibited least influence on the antibacterial effect of candidate probiotic while the other independent variables exhibited different degree of affect. The candidate probiotic revealed a reasonable antibacterial response in quite a wide range of temperature and pH in which the maximum levels were in the same range of optimum shrimp culture.

[1]  Zi-rong Xu,et al.  The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds , 2005, Fisheries Science.

[2]  K. Sonomoto,et al.  Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1: relationship between production of the lantibiotic and lactate and cell growth , 1996, Applied Microbiology and Biotechnology.

[3]  J. Balcázar,et al.  Effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (Litopenaeus vannamei) following immersion challenge with Vibrio parahaemolyticus. , 2007, Journal of invertebrate pathology.

[4]  Yanbo Wang.  Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei , 2007 .

[5]  S. K. Pandian,et al.  Screening and evaluation of probiotics as a biocontrol agent against pathogenic Vibrios in marine aquaculture , 2007, Letters in applied microbiology.

[6]  H. Kaiser,et al.  Probiotics in marine larviculture. , 2006, FEMS microbiology reviews.

[7]  Zi-rong Xu,et al.  Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities , 2006 .

[8]  M. Burke,et al.  AQUACULTURE AND OCEAN RESOURCES: Raising Tigers of the Sea , 2005 .

[9]  Quanhong Li,et al.  Application of response surface methodology for extraction optimization of germinant pumpkin seeds protein , 2005 .

[10]  K. Nealson,et al.  Low-Temperature Growth of Shewanella oneidensis MR-1 , 2005, Applied and Environmental Microbiology.

[11]  R. Naylor,et al.  Future seascapes, fishing, and fish farming , 2005 .

[12]  B. Pettersson,et al.  DNA based classification of food associated Enterobacteriaceae previously identified by Biolog GN Microplates. , 2004, Systematic and applied microbiology.

[13]  A. Boxall,et al.  Veterinary medicines in the environment. , 2004, Reviews of environmental contamination and toxicology.

[14]  E. Parente,et al.  Influence of pH on growth and bacteriocin production byLactococcus lactis subsp.lactis 14ONWC during batch fermentation , 2004, Applied Microbiology and Biotechnology.

[15]  B. Vaseeharan,et al.  Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon , 2003, Letters in applied microbiology.

[16]  B. Austin,et al.  Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum) , 2002 .

[17]  I. Karunasagar,et al.  Inhibition of shrimp pathogenic vibrios by a marine Pseudomonas I-2 strain , 2002 .

[18]  C. I. Chang,et al.  An evaluation of two probiotic bacterial strains, Enterococcus faecium SF68 and Bacillus toyoi, for reducing edwardsiellosis in cultured European eel, Anguilla anguilla L. , 2002 .

[19]  M. Shariff,et al.  The effectiveness of a commercial microbial product in poorly prepared tiger shrimp, Penaeus monodon (Fabricius), ponds , 2001 .

[20]  B. D. Chang,et al.  Environmental impact of chemical wastes produced by the salmon aquaculture industry , 2001 .

[21]  B. Gómez‐Gil,et al.  The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms , 2000 .

[22]  O. Sharma,et al.  Effect of Aquazyn-TM-1000, a probiotic on the water quality and growth of Cyprinus carpio var. communis (L.) , 2000 .

[23]  K. Nealson,et al.  Shewanella pealeana sp. nov., a member of the microbial community associated with the accessory nidamental gland of the squid Loligo pealei. , 1999, International journal of systematic bacteriology.

[24]  F. Gatesoupe.  The use of probiotics in aquaculture , 1999 .

[25]  Jorunn Skjermo,et al.  Techniques for microbial control in the intensive rearing of marine larvae , 1999 .

[26]  P. Germain,et al.  Influence of temperature and pH on production of two bacteriocins by Leuconostoc mesenteroides subsp. mesenteroides FR52 during batch fermentation , 1998, Applied Microbiology and Biotechnology.

[27]  D. Moriarty.  Control of luminous Vibrio species in penaeid aquaculture ponds , 1998 .

[28]  P. B. E. Trevino,et al.  Bailey and Scott's Diagnostic Microbiology , 1998 .

[29]  S. Kjelleberg,et al.  Colonization in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. strain K1 , 1997 .

[30]  J. Nissen-Meyer,et al.  Production and pH-Dependent Bactericidal Activity of Lactocin S, a Lantibiotic from Lactobacillus sake L45 , 1995, Applied and environmental microbiology.

[31]  E. Parente,et al.  Influence of pH on the production of enterocin 1146 during batch fermentation , 1994, Letters in applied microbiology.

[32]  E. Parente,et al.  Influence of pH on growth and bacteriocin production byLactococcus lactis subsp.lactis 14ONWC during batch fermentation , 1994 .

[33]  T. Chua,et al.  Shrimp Culture: Pond Design, Operation and Management , 1986 .

[34]  E. Baron,et al.  Bailey and Scott's Diagnostic microbiology , 1982 .