Near-Infrared Light Triggered Upconversion Optogenetic Nanosystem for Cancer Therapy.

In vivo the application of optogenetic manipulation in deep tissue is seriously obstructed by the limited penetration depth of visible light that is continually applied to activate a photoactuator. Herein, we designed a versatile upconversion optogenetic nanosystem based on a blue-light-mediated heterodimerization module and rare-earth upconversion nanoparticles (UCNs). The UCNs worked as a nanotransducer to convert external deep-tissue-penetrating near-infrared (NIR) light to local blue light to noninvasively activate photoreceptors for optogenetic manipulation in vivo. In this, we demonstrated that deeply penetrating NIR light could be used to control the apoptotic signaling pathway of cancer cells in both mammalian cells and mice by UCNs. We believe that this interesting NIR-light-responsive upconversion optogenetic nanotechnology has significant application potentials for both basic research and clinical applications in vivo.

[1]  Zhenyu Liao,et al.  A visual guide to gene/optothermal synergy therapy nanosystem using tungsten oxide. , 2017, Journal of colloid and interface science.

[2]  Hans-Jürgen Butt,et al.  Near‐Infrared‐Sensitive Materials Based on Upconverting Nanoparticles , 2016, Advanced materials.

[3]  Zhen Gu,et al.  A Two-Stage Dissociation System for Multilayer Imaging of Cancer Biomarker-Synergic Networks in Single Cells. , 2017, Angewandte Chemie.

[4]  P. Bornstein,et al.  TOM22, a core component of the mitochondria outer membrane protein translocation pore, is a mitochondrial receptor for the proapoptotic protein Bax , 2007, Cell Death and Differentiation.

[5]  P. Quail,et al.  Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light , 1999, Nature.

[6]  Renren Deng,et al.  Tuning upconversion through energy migration in core-shell nanoparticles. , 2011, Nature materials.

[7]  Wei Huang,et al.  Temporal full-colour tuning through non-steady-state upconversion. , 2015, Nature nanotechnology.

[8]  D. Goldfarb,et al.  Importin alpha: a multipurpose nuclear-transport receptor. , 2004, Trends in cell biology.

[9]  Quanyin Hu,et al.  Relay Drug Delivery for Amplifying Targeting Signal and Enhancing Anticancer Efficacy , 2017, Advanced materials.

[10]  Christopher A. Voigt,et al.  Spatiotemporal Control of Cell Signalling Using A Light-Switchable Protein Interaction , 2009, Nature.

[11]  David S Lawrence,et al.  Optogenetic apoptosis: light-triggered cell death. , 2015, Angewandte Chemie.

[12]  Sarah Hurst Petrosko,et al.  Accelerating the Translation of Nanomaterials in Biomedicine. , 2015, ACS nano.

[13]  Kongchang Wei,et al.  Remote Control of Multimodal Nanoscale Ligand Oscillations Regulates Stem Cell Adhesion and Differentiation. , 2017, ACS nano.

[14]  Daisuke Kohda,et al.  Functions of outer membrane receptors in mitochondrial protein import. , 2002, Biochimica et biophysica acta.

[15]  Muthu Kumara Gnanasammandhan,et al.  In vivo photodynamic therapy using upconversion nanoparticles as remote-controlled nanotransducers , 2012, Nature Medicine.

[16]  Ludovic Jullien,et al.  How to control proteins with light in living systems. , 2014, Nature chemical biology.

[17]  M. Ehlers,et al.  Rapid blue light induction of protein interactions in living cells , 2010, Nature Methods.

[18]  Vladislav V Verkhusha,et al.  An optogenetic system based on bacterial phytochrome controllable with near-infrared light , 2016, Nature Methods.

[19]  Petras Juzenas,et al.  Noninvasive fluorescence excitation spectroscopy during application of 5-aminolevulinic acid in vivo , 2002, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[20]  Yuta Nihongaki,et al.  Photoactivatable CRISPR-Cas9 for optogenetic genome editing , 2015, Nature Biotechnology.

[21]  Won Do Heo,et al.  Reversible protein inactivation by optogenetic trapping in cells , 2014, Nature Methods.

[22]  Jun Lin,et al.  Enhanced Antitumor Efficacy by 808 nm Laser‐Induced Synergistic Photothermal and Photodynamic Therapy Based on a Indocyanine‐Green‐Attached W18O49 Nanostructure , 2015 .

[23]  Moritoshi Sato,et al.  Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins , 2015, Nature Communications.

[24]  Xueyuan Chen,et al.  Upconversion nanoparticles in biological labeling, imaging, and therapy. , 2010, The Analyst.

[25]  J. Blenis,et al.  Death Receptor Recruitment of Endogenous Caspase-10 and Apoptosis Initiation in the Absence of Caspase-8* , 2001, The Journal of Biological Chemistry.

[26]  J. Camonis,et al.  A Novel Protein That Interacts with the Death Domain of Fas/APO1 Contains a Sequence Motif Related to the Death Domain (*) , 1995, The Journal of Biological Chemistry.

[27]  D. Goldfarb,et al.  Importin α: A multipurpose nuclear-transport receptor , 2004 .

[28]  Vasilis Ntziachristos,et al.  Shedding light onto live molecular targets , 2003, Nature Medicine.

[29]  Yong Zhang,et al.  Upconversion fluorescent nanoparticles as a potential tool for in-depth imaging , 2011, Nanotechnology.

[30]  Ying Zhang,et al.  NIR‐Remote Selected Activation Gene Expression in Living Cells by Upconverting Microrods , 2016, Advanced materials.

[31]  M. Peter,et al.  Cytotoxicity‐dependent APO‐1 (Fas/CD95)‐associated proteins form a death‐inducing signaling complex (DISC) with the receptor. , 1995, The EMBO journal.

[32]  Sangsik Lee,et al.  The roles of FADD in extrinsic apoptosis and necroptosis. , 2012, BMB reports.

[33]  L. Epstein,et al.  In situ trypan blue staining of monolayer cell cultures for permanent fixation and mounting. , 1997, BioTechniques.

[34]  Xiaogang Liu,et al.  Recent Advances in the Chemistry of Lanthanide‐Doped Upconversion Nanocrystals , 2009 .

[35]  H. Adesnik,et al.  Optogenetic pharmacology for control of native neuronal signaling proteins , 2013, Nature Neuroscience.

[36]  Jin Chang,et al.  Persistent Luminescent Nanocarrier as an Accurate Tracker in Vivo for Near Infrared-Remote Selectively Triggered Photothermal Therapy. , 2016, ACS applied materials & interfaces.

[37]  Jin Chang,et al.  A NIR-remote controlled upconverting nanoparticle: an improved tool for living cell dye-labeling. , 2015, Nanotechnology.

[38]  Orion D. Weiner,et al.  Illuminating cell signalling with optogenetic tools , 2014, Nature Reviews Molecular Cell Biology.

[39]  K. Gardner,et al.  An optogenetic gene expression system with rapid activation and deactivation kinetics , 2013, Nature chemical biology.

[40]  Helmut Schäfer,et al.  Upconverting nanoparticles. , 2011, Angewandte Chemie.