LOCALIZATION OF SODIUM PUMPS IN THE CHOROID PLEXUS EPITHELIUM

The ability of epithelial membranes to absorb and secrete salt and water is a subject of considerable physiological importance . Although the detailed mechanisms involved are not understood, it is generally accepted that active sodium transport is the driving force for fluid movement across the intestine, gall bladder, renal tubule, and choroid plexus, and that sodium pumping is linked to the activity of membrane-bound Na/K ATPases (see Bonting, 1970) . Furthermore, it is well established that cardiac glycosides, like ouabain, are potent and specific inhibitors of these enzymes and sodium transport. Thus, cytological localization of the site of ouabain action should provide strong evidence as to the location of sodium pumps in epithelia . This report describes a combined biochemical and autoradiographic approach to localization of

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