Marked temperature dependence of the platelet calcium signal induced by human von Willebrand factor.

Interaction of von Willebrand factor (vWF) with the platelet is essential to hemostasis when vascular injury occurs. This interaction elevates the intracellular free calcium concentration ([Ca2+]i) and promotes platelet activation. The present study investigated the temperature dependence of vWF-induced [Ca2+]i signaling in human platelets. The influence of temperature can provide invaluable insight into the underlying mechanism. Platelet [Ca2+]i was monitored with Fura-PE3. Ristocetin-mediated binding of vWF induced a transient platelet [Ca2+]i increase at 37 degrees C, but no response at lower temperatures (20 degrees C to 25 degrees C). This temperature dependence could not be attributed to a reduction in vWF binding, as ristocetin-mediated platelet aggregation and agglutination were essentially unaffected by temperature. Most other platelet agonists (U-46619, alpha-thrombin, and adenosine 5'-diphosphate [ADP]) induced a [Ca2+]i signal whose amplitude did not diminish at lower temperatures. The [Ca2+]i signal in response to arachidonic acid, however, showed similar temperature dependence to that seen with vWF. Assessment of thromboxane A2 production showed a strong temperature dependence for metabolism of arachidonic acid by the cyclo-oxygenase pathway. vWF induced thromboxane A2 production in the platelet. Aspirin treatment abolished the vWF-induced [Ca2+]i signal. These observations suggest that release of arachidonic acid and its conversion to thromboxane A2 play a central role in vWF-mediated [Ca2+]i signaling in the platelet at physiological temperatures.

[1]  R. Kulmacz.  Cellular regulation of prostaglandin H synthase catalysis , 1998, FEBS letters.

[2]  E. Niggli,et al.  Confocal near-membrane detection of calcium in cardiac myocytes. , 1998, Cell calcium.

[3]  Y. Usson,et al.  Nuclear and cytoplasmic free calcium level changes induced by elastin peptides in human endothelial cells. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. Kermode,et al.  Ristocetin-mediated interaction of human von Willebrand factor with platelet glycoprotein lb evokes a transient calcium signal: observations with Fura-PE3. , 1998, Journal of Laboratory and Clinical Medicine.

[5]  J. Kermode,et al.  Ratiometric Measurements with Fluorescent Indicators Should Be Interpreted Cautiously in Studies of Platelet Calcium Signaling for von Willebrand Factor , 1997, Thrombosis and Haemostasis.

[6]  Peter Lipp,et al.  Cooking with Calcium: The Recipes for Composing Global Signals from Elementary Events , 1997, Cell.

[7]  K. Furuya,et al.  Temperature dependency of calcium responses in mammary tumour cells , 1997, Cell biochemistry and function.

[8]  J. Cazenave,et al.  Platelet ADP/Purinergic Receptors , 1997 .

[9]  J. Moake,et al.  Shear stress-induced binding of von Willebrand factor to platelets. , 1997, Biorheology.

[10]  José A López,et al.  Molecular mechanisms of platelet adhesion and activation. , 1997, The international journal of biochemistry & cell biology.

[11]  W. Graier,et al.  Temperature dependence of agonist-stimulated Ca2+ signaling in cultured endothelial cells. , 1997, Cell calcium.

[12]  E. Cook,et al.  Fluorescent indicators give biased estimates of intracellular free calcium change in aggregating platelets: implication for studies with human von Willebrand factor. , 1996, Blood cells, molecules & diseases.

[13]  Shingo Nakatani,et al.  Detection of Platelet Adhesion/Aggregation to Immobilized Ligands on Microbeads by an Aggregometer , 1996, Thrombosis and Haemostasis.

[14]  R. Deana,et al.  Inhibitory Effect of Prostacyclin and Nitroprusside on Type IIB von Willebrand Factor-promoted Platelet Activation , 1996, Thrombosis and Haemostasis.

[15]  L. De Marco,et al.  Porcine von Willebrand Factor Binding to Human Platelet GPIb Induces Transmembrane Calcium Influx , 1996, Thrombosis and Haemostasis.

[16]  J. Moake,et al.  Platelets and shear stress. , 1996, Blood.

[17]  R. Deana,et al.  Type IIB von Willebrand factor induces phospholipase A2 activation and cytosolic Ca2+ increase in platelets. , 1995, Biochemical and biophysical research communications.

[18]  J. Brugge,et al.  Integrins and signal transduction pathways: the road taken. , 1995, Science.

[19]  Y. Yatomi,et al.  Protein tyrosine phosphorylation in human platelets induced by interaction between glycoprotein Ib and von Willebrand factor. , 1995, Biochimica et biophysica acta.

[20]  E. Neer.  Heterotrimeric C proteins: Organizers of transmembrane signals , 1995, Cell.

[21]  R. Deana,et al.  Platelet Aggregation Induced by Plasma from Type IIB von Willebrand’s Disease Patients Is Associated with an Increase in Cytosolic Ca2+ Concentration , 1993, Thrombosis and Haemostasis.

[22]  U. Martinowitz,et al.  Platelet Aggregation on Extracellular Matrix: Effect of a Recombinant GPIb-Binding Fragment of von Willebrand Factor , 1993, Thrombosis and Haemostasis.

[23]  A. Aviv,et al.  Kinetics of calcium transport across the lymphocyte plasma membrane. , 1993, The American journal of physiology.

[24]  Z. Ruggeri,et al.  Mechanisms of Shear-induced Platelet Adhesion and Aggregation , 1993, Thrombosis and Haemostasis.

[25]  Y. Ikeda,et al.  Transmembrane Calcium Influx Associated with von Willebrand Factor Binding to GP Ib in the Initiation of Shear-Induced Platelet Aggregation , 1993, Thrombosis and Haemostasis.

[26]  M. Scrutton,et al.  The Platelet as a Cа2+-Driven Cell: Mechanisms Which May Modulate Cа2+-Driven Responses , 1993 .

[27]  J. Heemskerk,et al.  Calcium influx mechanisms and signal organisation in human platelets. , 1993, Advances in experimental medicine and biology.

[28]  L. V. van Tits,et al.  Dissociation between phytohaemagglutinin-stimulated generation of inositol phosphates and Ca2+ increase in human mononuclear leucocytes. , 1992, The Biochemical journal.

[29]  B. Savage,et al.  Modulation of platelet function through adhesion receptors. A dual role for glycoprotein IIb-IIIa (integrin alpha IIb beta 3) mediated by fibrinogen and glycoprotein Ib-von Willebrand factor. , 1992, The Journal of biological chemistry.

[30]  J. Moake,et al.  Shear stress-induced von Willebrand factor binding to platelet glycoprotein Ib initiates calcium influx associated with aggregation. , 1992, Blood.

[31]  J. García-Sancho,et al.  Agonist-induced Ca2+ influx into human platelets is secondary to the emptying of intracellular Ca2+ stores. , 1991, The Biochemical journal.

[32]  J. Moake,et al.  von Willebrand factor binding to platelet GpIb initiates signals for platelet activation. , 1991, The Journal of clinical investigation.

[33]  R. Conroy,et al.  Heparin inhibition of von Willebrand factor-dependent platelet function in vitro and in vivo. , 1991, The Journal of clinical investigation.

[34]  J. Thompson,et al.  Effect of temperature on receptor-activated changes in [Ca2+]i and their determination using fluorescent probes. , 1991, The Journal of biological chemistry.

[35]  S. Mccarthy,et al.  Use of fluo-3 to measure cytosolic Ca2+ in platelets and neutrophils. Loading cells with the dye, calibration of traces, measurements in the presence of plasma, and buffering of cytosolic Ca2+. , 1990, The Biochemical journal.

[36]  Y. Ikeda,et al.  Change in intracellular calcium ions during shear induced platelet aggregation. , 1990, ASAIO transactions.

[37]  S. Sage,et al.  ADP evokes biphasic Ca2+ influx in fura-2-loaded human platelets. Evidence for Ca2+ entry regulated by the intracellular Ca2+ store. , 1990, The Biochemical journal.

[38]  M. Read,et al.  [12] Fixation platelets and platelet agglutination/aggregation tests , 1989 .

[39]  M. Read,et al.  Fixation platelets and platelet agglutination/aggregation tests. , 1989, Methods in enzymology.

[40]  J. Mustard,et al.  Isolation of human platelets from plasma by centrifugation and washing. , 1989, Methods in enzymology.

[41]  P. Weber,et al.  Prostaglandin endoperoxide analogues stimulate phospholipase C and protein phosphorylation during platelet shape change. , 1985, Blood.

[42]  S. Russell,et al.  Interaction of asialo von Willebrand factor with glycoprotein Ib induces fibrinogen binding to the glycoprotein IIb/IIIa complex and mediates platelet aggregation. , 1985, The Journal of clinical investigation.

[43]  R. Tsien,et al.  A new generation of Ca2+ indicators with greatly improved fluorescence properties. , 1985, The Journal of biological chemistry.

[44]  B. Coller,et al.  A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa. , 1983, The Journal of clinical investigation.

[45]  M. Buytenhek,et al.  Purification of PGH synthase from sheep vesicular glands. , 1982, Methods in enzymology.

[46]  V. Turitto,et al.  Effect of temperature on platelet interaction with subendothelium exposed to flowing blood. , 1974, Haemostasis.

[47]  M. Howard,et al.  Ristocetin - A New Tool in the Investigation of Platelet Aggregation , 1971, Thrombosis and Haemostasis.

[48]  M. Karpatkin,et al.  Methods for the Production of Clinically Effective Intermediate and High‐Purity Factor‐VIII Concentrates , 1971, British journal of haematology.