Staining of stratum corneum with fluorescent ε‐poly‐L‐lysine and its application to evaluation of skin conditions

ε‐Poly‐L‐lysine (PLL) is a cationic polymer consisting of 25 to 35 L‐lysine residues that adheres to the surface of skin as well as hair. However, the properties of PLL regarding its adhesion to the skin remain to be elucidated. In this study, we examined the staining of stratum corneum (SC) with fluorescence‐labeled PLL and explored its relationship with skin condition.

[1]  J. Mañes,et al.  Antimicrobial packaging based on ɛ‐polylysine bioactive film for the control of mycotoxigenic fungi in vitro and in bread , 2017, Journal of food processing and preservation.

[2]  T. Hirao.  Corneocyte Analysis , 2015 .

[3]  Y. Takema,et al.  Dry skin in the winter is related to the ceramide profile in the stratum corneum and can be improved by treatment with a Eucalyptus extract , 2013, Journal of cosmetic dermatology.

[4]  Takashi Kitahara,et al.  Comprehensive quantification of ceramide species in human stratum corneum[S] , 2009, Journal of Lipid Research.

[5]  A. Al-Amoudi,et al.  Stratum corneum keratin structure, function, and formation: the cubic rod-packing and membrane templating model. , 2004, The Journal of investigative dermatology.

[6]  H. Tagami,et al.  Improvement of Mild Inflammatory Changes of the Facial Skin Induced by Winter Environment with Daily Applications of a Moisturizing Cream , 2003, Dermatology.

[7]  H. Tagami,et al.  Ratio of immature cornified envelopes does not correlate with parakeratosis in inflammatory skin disorders , 2003, Experimental dermatology.

[8]  T. Hirao,et al.  Identification of immature cornified envelopes in the barrier‐impaired epidermis by characterization of their hydrophobicity and antigenicities of the components , 2001, Experimental dermatology.

[9]  T. Hirao,et al.  Elevation of interleukin 1 receptor antagonist in the stratum corneum of sun-exposed and ultraviolet B-irradiated human skin. , 1996, The Journal of investigative dermatology.

[10]  H. Tagami,et al.  Stratum corneum hydration and amino acid content in xerotic skin , 1989, The British journal of dermatology.

[11]  H. Sakai,et al.  Antimicrobial action of epsilon-poly-L-lysine. , 1984, The Journal of antibiotics.

[12]  J. Barrett,et al.  Histidine-rich protein of the keratohyalin granules. Source of the free amino acids, urocanic acid and pyrrolidone carboxylic acid in the stratum corneum. , 1982, Biochimica et biophysica acta.

[13]  D. Roberts,et al.  The determination of regional and age variations in the rate of desquamation: a comparison of four techniques. , 1980, The Journal of investigative dermatology.

[14]  G. Plewig,et al.  Effects of dermatitis, stripping, and steroids on the morphology of corneocytes. A new bioassay. , 1977, The Journal of investigative dermatology.