Inhibitory factors involved in acetone-butanol fermentation byClostridium saccharoperbutylacetonicum

Studies carried out onClostridium saccharoperbutylacetonicum (ATCC 27022) reveal that intracellular and extracellular inhibitors, including metabolic end-products, caused the inhibition of cell growth and solvent production. Butanol at the level of 13.0 g/liter was completely inhibitory to the growth of cells, whereas butyric acid totally inhibited the cell growth at a concentration of 8.7 g/liter. Investigations carried out on the effect of addition of culture filtrate concentrate and cell-free extract concentrate indicate that nonvolatile inhibitors produced by cells were also inhibitory for bioconversion. The butanol production was found to be reduced by 15%–20% on addition of cell-free extract concentrate. Inhibition by concentrates was enhanced in the presence of butanol. Addition of heat-sterilized concentrates resulted in a reduction of inhibition.

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