An investigation on fluorescent-optical dual-mode tomography using time-resolved data

We present a scheme for fluorescence guided diffusion optical tomography to reconstruct the fluorescence parameters (yield and lifetime) and optical parameters (absorption and reduced coefficients) based on time-resolved data. In this paper, the fluorescence parameters were reconstructed at first, then the fluorescence images were used to guide and constrain the diffusion optical tomography reconstruction, and the binary image segmentation strategy was applied to improve the image quality in DOT. To validate the proposed method, the numerical simulation was performed to demonstrate its computational efficacy. The results showed the feasibility of this method, and the spatial resolution, quantification and computational efficiency in DOT were enhanced evidently.

[1]  N. Otsu.  A threshold selection method from gray level histograms , 1979 .

[2]  Andrew K. Dunn,et al.  A Time Domain Fluorescence Tomography System for Small Animal Imaging , 2008, IEEE Transactions on Medical Imaging.

[3]  Vasilis Ntziachristos,et al.  Accuracy of fluorescent tomography in the presence of heterogeneities:study of the normalized born ratio , 2005, IEEE Transactions on Medical Imaging.

[4]  Huijuan Zhao,et al.  Three-dimensional scheme for time-domain fluorescence molecular tomography based on Laplace transforms with noise-robust factors. , 2008, Optics express.

[5]  Eva M Sevick-Muraca,et al.  Fluorescence-enhanced, near infrared diagnostic imaging with contrast agents. , 2002, Current opinion in chemical biology.

[6]  Gregory Boverman,et al.  Time resolved fluorescence tomography of turbid media based on lifetime contrast. , 2006, Optics express.

[7]  P M Schlag,et al.  Assessment of the size, position, and optical properties of breast tumors in vivo by noninvasive optical methods. , 1998, Applied optics.

[8]  Mark A A Neil,et al.  Fluorescence lifetime imaging by using time-gated data acquisition. , 2007, Applied optics.

[9]  S R Arridge,et al.  Recent advances in diffuse optical imaging , 2005, Physics in medicine and biology.

[10]  Rinaldo Cubeddu,et al.  Combined reconstruction of fluorescent and optical parameters using time-resolved data. , 2009, Applied optics.

[11]  Simon R Cherry,et al.  In vivo molecular and genomic imaging: new challenges for imaging physics. , 2004, Physics in medicine and biology.