Molecular analyses of Cordyceps gunnii in China

Cordyceps gunnii Berk., originally named for a specimen discovered in Tasmania, Australia, is commonly reported in China as an adulterant of the valuable Chinese medicinal fungus, Cordyceps sinensis. Correct identification of this adulterant species bears close relevance to herb control, legal disputes and forensic measures. Analyses of the internal transcribed spacers of the nuclear ribosomal DNA repeats (ITS) and three loci [nuclear ribosomal large subunit (nrLSU), elongation factor 1α (EF-1α) and the largest subunit of RNA polymerase Ⅱ (rpb1)] of C. gunnii from China and from Tasmania revealed that they are not conspecific. The Cordyceps species recognized as C. gunnii in China is clustered with those species that are regarded as belonging to the genus Metacordyceps and placed in the Clavicipitaceae family. Tasmanian samples, however, are closer to C. sinensis and belong to the genus Ophiocordyceps in the Ophiocordycipitaceae family. Re-investigation of the taxonomic status of this adulterant fungus is urgently needed to avoid unnecessary dispute. At this stage, reference to the Chinese samples of Cordyceps gunnii can be tentatively labeled as Cordyceps gunnii auctorum non Berkeley (Cordyceps gunnii auct. non Berk.). The tested sequences can also be useful for differentiating the adulterant 'Cordyceps gunnii' from genuine samples of Cordyceps sinensis.

[1]  Yanjiao Zhou,et al.  Maturational Alteration of Oppositely Orientated rDNA and Differential Proliferation of GC- and AT-biased Genotypes of Ophiocordyceps sinensis and Paecilomyces hepiali in Natural Cordyceps sinensis 1 , 2010 .

[2]  Gi-Ho Sung,et al.  A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a combinational bootstrap approach. , 2007, Molecular phylogenetics and evolution.

[3]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[4]  J. Zhong,et al.  Secondary metabolites from Cordyceps species and their antitumor activity studies. , 2007, Recent patents on biotechnology.

[5]  J. Spatafora,et al.  Phylogenetic evidence for an animal pathogen origin of ergot and the grass endophytes , 2007, Molecular ecology.

[6]  J. Spatafora,et al.  Phylogenetic classification of Cordyceps and the clavicipitaceous fungi , 2007, Studies in mycology.

[7]  F. Q. Yang,et al.  Quality control of Cordyceps sinensis, a valued traditional Chinese medicine. , 2006, Journal of pharmaceutical and biomedical analysis.

[8]  P. Inglis,et al.  Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS Sequences , 2006 .

[9]  N. Hywel-Jones,et al.  Towards a phylogenetic classification of Cordyceps: ITS nrDNA sequence data confirm divergent lineages and paraphyly. , 2005, Mycological research.

[10]  Subrat Sharma.  Trade of Cordyceps sinensis from high altitudes of the Indian Himalaya: Conservation and biotechnological priorities , 2004 .

[11]  J. Spatafora,et al.  Multigene phylogeny reveals new lineage for Stachybotrys chartarum, the indoor air fungus. , 2004, Mycological research.

[12]  U. Yoon,et al.  A Rapid DNA Extraction Method for RFLP and PCR Analysis from a Single Dry Seed , 1998, Plant Molecular Biology Reporter.

[13]  W. Wan,et al.  Immunosuppressive activity of polysaccharides from Cordyceps gunnii mycelia in mice in vivo/vitro , 2004 .

[14]  Chienyan Hsieh,et al.  A comparison of the chemical composition and bioactive ingredients of the Chinese medicinal mushroom DongChongXiaCao, its counterfeit and mimic, and fermented mycelium of Cordyceps sinensis , 2002 .

[15]  Wang Ning,et al.  Differentiation of Medicinal Cordyceps Species by rDNA ITS Sequence Analysis , 2002, Planta medica.

[16]  M. Oborník,et al.  Phylogeny of mitosporic entomopathogenic fungi: is the genus Paecilomyces polyphyletic? , 2001, Canadian journal of microbiology.

[17]  R. Frutos,et al.  Insect Pathogens as Biological Control Agents: Do They Have a Future? , 2001 .

[18]  M. Nei,et al.  Molecular Evolution and Phylogenetics , 2000 .

[19]  T. A. Hall,et al.  BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT , 1999 .

[20]  Zeng-zhi Li,et al.  New Variety of Cordyceps gunnii (Berk.) Berk. and Its Paecilomyces Anamorph , 1999 .

[21]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[22]  R. Vilgalys,et al.  Ancient and recent patterns of geographic speciation in the oyster mushroom Pleurotus revealed by phylogenetic analysis of ribosomal DNA sequences. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[23]  Thomas J. White,et al.  PCR protocols: a guide to methods and applications. , 1990 .

[24]  J. Felsenstein.  CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP , 1985, Evolution; international journal of organic evolution.

[25]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[26]  Liang Zong-qi.  A DESCRIPTION OF CORDYCEPS GUNNII IN CHINA , 1983 .

[27]  E. Mains.  Species of Cordyceps Parasitic on Elaphomyces , 1957 .