SYNTHESIS , CHARACTERIZATION , IN VITRO ANTIPROLIFERATIVE AND ANTIMICROBIAL STUDY OF 3-METHYL-( 9 '-FLUORENE )-SPIRO-5-( 2 , 4-DITHIOHYDANTOIN )

This paper presents a method for synthesis, in vitro antiproliferative and antibacterial study of 3-methyl-(9'-fluorene)-spiro-5-(2,4-dithiohydantoin). The structure of the obtained product was investigated by UV-Vis, IR, FT-IR ATR and Raman spectroscopy. The anticancer effect of 3-methyl-(9'-fluorene)-spiro-5-(2,4-dithiohydantoin) was determined in both adherent and suspension cell lines originating from tumors in humans (A2058, ATCC and WERI-Rb-1). The cytotoxic effect was evaluated by WST-assay (Roche Applied Science). The antimicrobial activity of fluorenylspirohydantoin derivative against clinically isolated Gram-positive bacteria (Staphylococcus aureus ATCC 25923 and Bacillus subtilis), Gram-negative bacteria (Escherichia coli ATCC 25922, Salmonella enterica subsp enterica ATCC BAA-2162 and Pseudomonas aeruginosa ATCC 9027) and the yeast Candida albicans ATCC 10231 was also

[1]  Y. Feodorova,et al.  Synthesis and in vitro activity of platinum(II) complexes of two fluorenylspirohydantoins against a human tumour cell line , 2014, Biotechnology, biotechnological equipment.

[2]  Y. Feodorova,et al.  Synthesis, antimicrobial and in vitro antiproliferative activity of 4'- bromo-(9'-fluorene)-spiro-5-(2,4-dithiohydantoin) against tumor cells , 2013 .

[3]  M. Mitewa,et al.  Spectroscopic aspects of the coordination modes of 2,4-dithiohydantoins: Experimental and theoretical study on copper and nickel complexes of cyclohexanespiro-5-(2,4-dithiohydantoin) , 2010 .

[4]  M. Mitewa,et al.  Two cycloalkanespiro-5-(2-thiohydantoins): Synthesis, spectral and structural characterization , 2009 .

[5]  M. Mitewa,et al.  Synthesis and characterization of copper(II) and nickel(II) complexes of (9′-fluorene)-spiro-5-dithiohydantoin , 2008 .

[6]  G. Tyuliev,et al.  Copper complexes of two cycloalkanespiro-5-dithiohydantoins: Synthesis, oxidation states and characterization , 2008 .

[7]  M. Mitewa,et al.  Four cycloalkanespiro-4'-imidazolidine-2',5'-dithiones. , 2006, Acta crystallographica. Section C, Crystal structure communications.

[8]  Andy P. Monkman,et al.  White polymeric light-emitting diode based on a fluorene polymer/Ir complex blend system , 2005, Workshop on Building European OLED Infrastructure.

[9]  V. Enchev,et al.  Synthesis, Spectroscopic Characterization and ab initio Investigation of Thioanalogues of Spirohydantoins , 2005 .

[10]  E. Buyukbingol,et al.  Anti-cancer activity studies of indolalthiohydantoin (PIT) on certain cancer cell lines. , 2000, Farmaco.

[11]  M. Peterson,et al.  Spiro hydantoin aldose reductase inhibitors. , 1988, Journal of medicinal chemistry.

[12]  A. Kaminska,et al.  [Synthesis of benzylidene derivatives of 3-O-chlorophenyl-2-thiohydantoin and the study of their anticonvulsive properties]. , 1979, Acta poloniae pharmaceutica.

[13]  A. Szadowska,et al.  [Synthesis of benzylidene derivatives of 3-(m-chlorophenyl)-2-thiohydantoin and studies of their anticonvulsant action]. , 1978, Acta poloniae pharmaceutica.

[14]  A. Markovac,et al.  Hydantoins as antitumor agents. , 1977, Journal of medicinal chemistry.