Proton pump inhibitor (PPI) drugs are potent gastric acid suppressant medications used for the treatment of a variety of gastric-related disorders. PPIs induce a state clinically identical to atrophic gastritis, a leading cause of food-bound malabsorption of vitamin B12. Considering that absorption of food-bound vitamin B12 is dependent on gastric acid, there is a concern that long-term use of PPI drugs may lead to vitamin B12 depletion, however, the evidence is conflicting. Theoretically, the absorption of free vitamin B12 should continue undisrupted with PPI usage because it has no gastric acid requirement. The aim of this study was to examine the potential role of fortified food in preventing any depletion in vitamin B12 status owing to long-term usage of PPIs. Fortified food consumption and B-vitamin status were assessed in PPI users and in age-matched adults who were non-PPI users. Patients who had received PPI maintenance therapy for a period of >1 y were identified from a local GP practice, while non-PPI users were identified from a previous study to investigate B-vitamin intake and status in healthy male and female adults.