Improved transfection efficiency of an aliphatic lipid substituted 2 kDa polyethylenimine is attributed to enhanced nuclear association and uptake in rat bone marrow stromal cell

Lipid substitutions of cationic polymers are actively explored to enhance the efficiency of nonviral gene carriers. We recently took this approach to develop a novel gene carrier by grafting linoleic acid (LA) to relatively biocompatible 2 kDa polyethylenimine (PEI2). The resulting polymer (PEI2LA) displayed improved transfection efficiency over the unmodified PEI2. The intracellular kinetics and distribution of the respective polyplexes were investigated in the present study to gain a better understanding of the role of lipid modification in intracellular trafficking of gene carriers.

[1]  Kai Simons,et al.  Greasing their way: lipid modifications determine protein association with membrane rafts. , 2010, Biochemistry.

[2]  N. Yui,et al.  Intranuclear DNA release is a determinant of transfection activity for a non-viral vector: biocleavable polyrotaxane as a supramolecularly dissociative condenser for efficient intranuclear DNA release. , 2010, Biological & pharmaceutical bulletin.

[3]  M. Fukata,et al.  Protein palmitoylation in neuronal development and synaptic plasticity , 2010, Nature Reviews Neuroscience.

[4]  J. Feijen,et al.  Reducible poly(amido ethylenimine)-based gene delivery system for improved nucleus trafficking of plasmid DNA. , 2010, Bioconjugate chemistry.

[5]  G. Moseley,et al.  The efficiency of nuclear plasmid DNA delivery is a critical determinant of transgene expression at the single cell level , 2010, The journal of gene medicine.

[6]  Orawan Suwantong,et al.  Aliphatic lipid substitution on 2 kDa polyethylenimine improves plasmid delivery and transgene expression. , 2009, Molecular pharmaceutics.

[7]  H. Uludaǧ,et al.  Nonviral Delivery of Basic Fibroblast Growth Factor Gene to Bone Marrow Stromal Cells , 2009, Clinical orthopaedics and related research.

[8]  L. Shea,et al.  Efficacy of immobilized polyplexes and lipoplexes for substrate‐mediated gene delivery , 2009, Biotechnology and bioengineering.

[9]  Theerasak Rojanarata,et al.  Nuclear localization signal peptides enhance transfection efficiency of chitosan/DNA complexes delivery , 2008, International journal of pharmaceutics.

[10]  T. Chen,et al.  Intelligent Biosynthetic Nanobiomaterials (IBNs) for Hyperthermic Gene Delivery , 2008, Pharmaceutical Research.

[11]  Hasan Uludağ,et al.  Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers , 2008, BMC biotechnology.

[12]  Minhyung Lee,et al.  Dexamethasone-conjugated low molecular weight polyethylenimine as a nucleus-targeting lipopolymer gene carrier. , 2007, Bioconjugate chemistry.

[13]  Hideyoshi Harashima,et al.  Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic lipid‐mediated transgene expression , 2007, The journal of gene medicine.

[14]  Hiroyuki Mizuguchi,et al.  Quantitative and mechanism-based investigation of post-nuclear delivery events between adenovirus and lipoplex , 2007, Nucleic acids research.

[15]  Maryam Tabrizian,et al.  Effects of alginate inclusion on the vector properties of chitosan-based nanoparticles. , 2006, Journal of controlled release : official journal of the Controlled Release Society.

[16]  I. Khalil,et al.  Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery , 2006, Pharmacological Reviews.

[17]  C. Fong,et al.  Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[18]  M. Conese,et al.  Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes. , 2005, Molecular therapy : the journal of the American Society of Gene Therapy.

[19]  A. Göpferich,et al.  Polyethylenimine-based non-viral gene delivery systems. , 2005, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[20]  L. Kedes,et al.  The Ad5 fiber mediates nonviral gene transfer in the absence of the whole virus, utilizing a novel cell entry pathway , 2005, Gene Therapy.

[21]  D. Porteous,et al.  HIV-1 Tat protein transduction domain peptide facilitates gene transfer in combination with cationic liposomes. , 2004, Journal of controlled release : official journal of the Controlled Release Society.

[22]  Kadir Turan,et al.  In vitro characterization and delivery of chitosan-DNA microparticles into mammalian cells. , 2004, Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques.

[23]  Kazuo Maruyama,et al.  Transferrin-modified liposomes equipped with a pH-sensitive fusogenic peptide: an artificial viral-like delivery system. , 2004, Biochemistry.

[24]  Yuichi Yamasaki,et al.  In situ single cell observation by fluorescence resonance energy transfer reveals fast intra‐cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine , 2004, The journal of gene medicine.

[25]  L. Medina-Kauwe,et al.  Modulation of secretory functions in epithelia by adenovirus capsid proteins. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[26]  L. Medina-Kauwe.  Endocytosis of adenovirus and adenovirus capsid proteins. , 2003, Advanced drug delivery reviews.

[27]  T. Kissel,et al.  Integrin targeting using RGD‐PEI conjugates for in vitro gene transfer , 2003, The journal of gene medicine.

[28]  Kinam Park,et al.  Polycation gene delivery systems: escape from endosomes to cytosol , 2003, The Journal of pharmacy and pharmacology.

[29]  Mark A. Kay,et al.  Progress and problems with the use of viral vectors for gene therapy , 2003, Nature Reviews Genetics.

[30]  D. Bull,et al.  Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium , 2003, Gene Therapy.

[31]  H. Harashima,et al.  Visualization of intracellular trafficking of exogenous DNA delivered by cationic liposomes. , 2002, Biochemical and biophysical research communications.

[32]  T. Giorgio,et al.  Nuclear-associated plasmid, but not cell-associated plasmid, is correlated with transgene expression in cultured mammalian cells. , 2000, Molecular therapy : the journal of the American Society of Gene Therapy.

[33]  M. Cotten,et al.  Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus , 2000, Gene Therapy.

[34]  F R Haselton,et al.  Mitosis enhances transgene expression of plasmid delivered by cationic liposomes. , 1999, Biochimica et biophysica acta.

[35]  I. Maclachlan,et al.  Cationic lipid-mediated transfection of cells in culture requires mitotic activity , 1999, Gene Therapy.

[36]  G. Daum,et al.  Lipids of mitochondria. , 1985, Biochimica et biophysica acta.

[37]  Ernst Wagner,et al.  Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation. , 2002, Molecular therapy : the journal of the American Society of Gene Therapy.