c-erbB-2 overexpression and histologlical type of in situ and invasive breast carcinoma

Aims: To assess c-erbB-2 immunostaining in relation to morphological type of in situ and invasive breast carcinoma. Methods: Formalin fixed, wax embedded archival tissue was used. Invasive carcinomas comprised 50 infiltrating ductal (NOS); seven medullary, 10 tubular, 15 mucinous and 24 classic invasive lobular. In situ carcinomas comprised 48 ductal (DCIS) and 10 cases of lobular (LCIS). The antibodies used were pABI (poly-clonal) which stains cell lines that over express the c-erbB-2 oncogene, and ICR 12 (monoclonal) which stains sections of breast carcinoma known to show c-erbB-2 amplification. Results: Immunostaining consistent with c-erbB-2 overexpression was found in 10 out of50 cases ofinfiltrating ductal carcinoma (NOS), one of 24 infiltrating lobular carcinomas and one of seven medullary carcinomas only. Seventy per cent of ICR 12 positive cases of infiltrating ductal carcinoma also had extratumoral DCIS. Forty six per cent of pure DCIS lesions also showed strong membrane staining for c-erbB-2 protein, confined to large cell types. Conclusions: Immunostaining for c-erb

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