In-vitro biological studies of crude extracts and isolation of novel compound from Nymphaea pubescens leaf

Many diseases are associated with oxidative stress of accumulation of free radicals in the body. The aims of the present study were to evaluate in vitro antioxidants activities, anti-inflammatory and lethality as well as isolation of compound from that active crude extract of Nymphaea pubescens (Family Nymphaeaceae) leaf. The Hexane: Petroleum ether, Ethyl acetate and Methanol extracts of Nymphaea pubescens were used for DPPH, Super oxide scavenging Activity, Brine shrimp lethality method and 5-lipoxygenase inhibition and isolation of compound from methanol extract determined using column chromatography with NMR structural data. The tested plant extracts showed promising antioxidant and free radical scavenging activity, cytotoxicity and anti- inflammatory activity. The methanolic extract was partitioned with different solvent system by increasing their polarities (petroleum ether, n- hexane, methanol and ethyl acetate). The structure of the isolated compounds was established by extensive spectroscopic studies (IR, 1

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