Layer‐by‐Layer Encapsulation of Probiotics for Delivery to the Microbiome

The gastrointestinal (GI) microbiome is widely investigated for its role in many diseases. However, technologies designed for microbiome delivery are lacking. Here, a layer-by-layer (LbL) approach is reported for probiotic encapsulation to protect probiotics against GI tract insults and improve their adhesion and growth on the intestines. These advantages translate to significantly enhanced survival of LbL-probiotics in vivo.

[1]  E. Pamer.  Resurrecting the intestinal microbiota to combat antibiotic-resistant pathogens , 2016, Science.

[2]  C. Ash,et al.  Manipulating the Microbiota. , 2016, Science.

[3]  Thomas D Young,et al.  Tools for the Microbiome: Nano and Beyond. , 2015, ACS nano.

[4]  B. Strukelj,et al.  In vivo imaging of Lactococcus lactis, Lactobacillus plantarum and Escherichia coli expressing infrared fluorescent protein in mice , 2015, Microbial Cell Factories.

[5]  R. Langer,et al.  pH-responsive supramolecular polymer gel as an enteric elastomer for use in gastric devices , 2015, Nature materials.

[6]  F. Sonntag,et al.  A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents. , 2015, Lab on a chip.

[7]  Tommi Vatanen,et al.  The dynamics of the human infant gut microbiome in development and in progression toward type 1 diabetes. , 2015, Cell host & microbe.

[8]  D. Maresch,et al.  Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase , 2015, Microbial Cell Factories.

[9]  Eric J Alm,et al.  Host lifestyle affects human microbiota on daily timescales , 2014, Genome Biology.

[10]  S. Mitragotri,et al.  Mucoadhesive intestinal devices for oral delivery of salmon calcitonin. , 2013, Journal of controlled release : official journal of the Controlled Release Society.

[11]  Joseph J. Richardson,et al.  Immersive Polymer Assembly on Immobilized Particles for Automated Capsule Preparation , 2013, Advanced materials.

[12]  R. Schwabe,et al.  The Microbiome and Cancer , 2021, Gut Feelings.

[13]  J. Foster,et al.  Gut–brain axis: how the microbiome influences anxiety and depression , 2013, Trends in Neurosciences.

[14]  E. Zoetendal,et al.  Duodenal infusion of donor feces for recurrent Clostridium difficile. , 2013, The New England journal of medicine.

[15]  M. Blaser,et al.  Antibiotics in early life alter the murine colonic microbiome and adiposity , 2012, Nature.

[16]  G. Tzortzis,et al.  Microencapsulation of probiotics for gastrointestinal delivery. , 2012, Journal of controlled release : official journal of the Controlled Release Society.

[17]  P. Hammond.  Building biomedical materials layer-by-layer , 2012 .

[18]  M. Blaser,et al.  The human microbiome: at the interface of health and disease , 2012, Nature Reviews Genetics.

[19]  L. Fitzpatrick,et al.  Bacillus Coagulans GBI-30 (BC30) improves indices of Clostridium difficile-Induced colitis in mice , 2011, Gut pathogens.

[20]  A. Logan,et al.  Acne vulgaris, probiotics and the gut-brain-skin axis - back to the future? , 2011, Gut pathogens.

[21]  Frank Caruso,et al.  Layer-by-layer-assembled capsules and films for therapeutic delivery. , 2010, Small.

[22]  Hsin‐Lung Chen,et al.  Enteric-coated capsules filled with freeze-dried chitosan/poly(gamma-glutamic acid) nanoparticles for oral insulin delivery. , 2010, Biomaterials.

[23]  S. Rokka,et al.  Protecting probiotic bacteria by microencapsulation: challenges for industrial applications , 2010 .

[24]  R. Sleator,et al.  Probiotics and gastrointestinal disease: successes, problems and future prospects , 2009, Gut pathogens.

[25]  R. Knight,et al.  The Effect of Diet on the Human Gut Microbiome: A Metagenomic Analysis in Humanized Gnotobiotic Mice , 2009, Science Translational Medicine.

[26]  P. Dettmar,et al.  The role of an alginate suspension on pepsin and bile acids – key aggressors in the gastric refluxate. Does this have implications for the treatment of gastro‐oesophageal reflux disease? , 2009 .

[27]  Larysa Hun.  Original Research: Bacillus coagulans Significantly Improved Abdominal Pain and Bloating in Patients with IBS , 2009, Postgraduate medicine.

[28]  F. Mégraud,et al.  Gut Pathogens: enteric health at the interface of changing microbiology , 2009, Gut pathogens.

[29]  N. Shah,et al.  Acid, bile, and heat tolerance of free and microencapsulated probiotic bacteria. , 2007, Journal of food science.

[30]  C. Press.  Cell host & microbe , 2007 .

[31]  E. Mardis,et al.  An obesity-associated gut microbiome with increased capacity for energy harvest , 2006, Nature.

[32]  Yves-Jacques Schneider,et al.  Nanoparticles as potential oral delivery systems of proteins and vaccines: a mechanistic approach. , 2006, Journal of controlled release : official journal of the Controlled Release Society.

[33]  T. E. Abraham,et al.  Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan--a review. , 2006, Journal of controlled release : official journal of the Controlled Release Society.

[34]  M. Elimelech,et al.  Aggregation kinetics of alginate-coated hematite nanoparticles in monovalent and divalent electrolytes. , 2006, Environmental science & technology.

[35]  C. Howden,et al.  Review article: immediate‐release proton‐pump inhibitor therapy – potential advantages , 2005, Alimentary pharmacology & therapeutics.

[36]  J. Smart.  The basics and underlying mechanisms of mucoadhesion. , 2005, Advanced drug delivery reviews.

[37]  E. Purdom,et al.  Diversity of the Human Intestinal Microbial Flora , 2005, Science.

[38]  A. Bernkop‐Schnürch,et al.  Improvement in the mucoadhesive properties of alginate by the covalent attachment of cysteine. , 2001, Journal of controlled release : official journal of the Controlled Release Society.

[39]  A. Deschamps,et al.  Acid and bile tolerance of spore-forming lactic acid bacteria. , 2000, International journal of food microbiology.

[40]  M. Fick,et al.  Lactic acid production by Bacillus coagulans—kinetic studies and optimization of culture medium for batch and continuous fermentations , 1999 .

[41]  J F Barrett,et al.  Structure, function, and assembly of cell walls of gram-positive bacteria. , 1983, Annual review of microbiology.

[42]  F Theeuwes,et al.  Elementary osmotic pump. , 1975, Journal of pharmaceutical sciences.

[43]  D. Small,et al.  Detergent properties of bile salts: correlation with physiological function. , 1967, Annual review of medicine.