Outside looking in: nanotube transistor intracellular sensors.

Nanowire-based field-effect transistors, including devices with planar and three-dimensional configurations, are being actively explored as detectors for extra- and intracellular recording due to their small size and high sensitivities. Here we report the synthesis, fabrication, and characterization of a new needle-shaped nanoprobe based on an active silicon nanotube transistor, ANTT, that enables high-resolution intracellular recording. In the ANTT probe, the source/drain contacts to the silicon nanotube are fabricated on one end, passivated from external solution, and then time-dependent changes in potential can be recorded from the opposite nanotube end via the solution filling the tube. Measurements of conductance versus water-gate potential in aqueous solution show that the ANTT probe is selectively gated by potential changes within the nanotube, thus demonstrating the basic operating principle of the ANTT device. Studies interfacing the ANTT probe with spontaneously beating cardiomyocytes yielded stable intracellular action potentials similar to those reported by other electrophysiological techniques. In addition, the straightforward fabrication of ANTT devices was exploited to prepare multiple ANTT structures at the end of single probes, which enabled multiplexed recording of intracellular action potentials from single cells and multiplexed arrays of single ANTT device probes. These studies open up unique opportunities for multisite recordings from individual cells through cellular networks.

[1]  B Sakmann,et al.  Patch clamp techniques for studying ionic channels in excitable membranes. , 1984, Annual review of physiology.

[2]  Hao Yan,et al.  Programmable nanowire circuits for nanoprocessors , 2011, Nature.

[3]  B. Cui,et al.  Intracellular Recording of Action Potentials by Nanopillar Electroporation , 2012, Nature nanotechnology.

[4]  Dongmok Whang,et al.  Nanolithography Using Hierarchically Assembled Nanowire Masks , 2003 .

[5]  Charles M. Lieber,et al.  Epitaxial core–shell and core–multishell nanowire heterostructures , 2002, Nature.

[6]  Charles M. Lieber,et al.  Design and Implementation of Functional Nanoelectronic Interfaces With Biomolecules, Cells, and Tissue Using Nanowire Device Arrays , 2010, IEEE Transactions on Nanotechnology.

[7]  Hao Yan,et al.  Layer-by-layer assembly of nanowires for three-dimensional, multifunctional electronics. , 2007, Nano letters.

[8]  T. Bryllert,et al.  Vertical high-mobility wrap-gated InAs nanowire transistor , 2006, IEEE Electron Device Letters.

[9]  W. Rutten.  Selective electrical interfaces with the nervous system. , 2002, Annual review of biomedical engineering.

[10]  Charles M Lieber,et al.  Large-area blown bubble films of aligned nanowires and carbon nanotubes. , 2007, Nature nanotechnology.

[11]  Peidong Yang,et al.  Silicon Vertically Integrated Nanowire Field Effect Transistors , 2006 .

[12]  F. Patolsky,et al.  Shape- and dimension-controlled single-crystalline silicon and SiGe nanotubes: toward nanofluidic FET devices. , 2009, Journal of the American Chemical Society.

[13]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[14]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .

[15]  Charles M. Lieber,et al.  Three-Dimensional, Flexible Nanoscale Field-Effect Transistors as Localized Bioprobes , 2010, Science.

[16]  Charles M Lieber,et al.  Synthetically encoded ultrashort-channel nanowire transistors for fast, pointlike cellular signal detection. , 2012, Nano letters.

[17]  Gengfeng Zheng,et al.  Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species , 2006, Nature Protocols.

[18]  R. D. Purves,et al.  Microelectrode methods for intracellular recording and ionophoresis , 1981 .

[19]  J. Shappir,et al.  In-cell recordings by extracellular microelectrodes , 2010, Nature Methods.

[20]  Michael Brecht,et al.  Electrophysiological recordings from behaving animals—going beyond spikes , 2009, Current Opinion in Neurobiology.

[21]  C. Wilkinson,et al.  An extracellular microelectrode array for monitoring electrogenic cells in culture. , 1990, Biosensors & bioelectronics.

[22]  Jacob T. Robinson,et al.  Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits. , 2012, Nature nanotechnology.

[23]  Brian Paul Timko.  Semiconductor nanowire devices: Novel morphologies and applications to electrogenic biological systems , 2009 .

[24]  F. Patolsky,et al.  Wall-selective chemical alteration of silicon nanotube molecular carriers. , 2011, Journal of the American Chemical Society.

[25]  Charles M Lieber,et al.  Kinked p-n junction nanowire probes for high spatial resolution sensing and intracellular recording. , 2012, Nano letters.

[26]  Tristan Barrett,et al.  Simultaneous multicolor imaging of five different lymphatic basins using quantum dots. , 2007, Nano letters.

[27]  D. Bers.  Cardiac excitation–contraction coupling , 2002, Nature.

[28]  Charles M. Lieber,et al.  Semiconductor nanowires: A platform for nanoscience and nanotechnology , 2010, 2010 3rd International Nanoelectronics Conference (INEC).

[29]  Charles M Lieber,et al.  Flexible electrical recording from cells using nanowire transistor arrays , 2009, Proceedings of the National Academy of Sciences.

[30]  C. Lieber,et al.  Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species , 2001, Science.

[31]  J. Jalife,et al.  Cardiac Electrophysiology: From Cell to Bedside , 1990 .

[32]  Bozhi Tian,et al.  Intracellular recordings of action potentials by an extracellular nanoscale field-effect transistor , 2011, Nature nanotechnology.