Lateral flow biosensor for DNA extraction-free detection of Salmonella based on aptamer mediated strand displacement amplification.

Convenient and sensitive point-of-care rapid diagnostic tests for food-borne pathogens have been a long-felt need of clinicians. Traditional approaches such as culture-based methods have good sensitivity and specificity, but they tend to be tedious and time-consuming. Herein we present a simple and sensitive aptamer based biosensor for rapid detection of Salmonella enteritidis (S. enteritidis). One of the aptamers specific for the outmembrane of S. enteritidis was used for magnetic bead enrichments. Another aptamer against S. enteritidis was used as a reporter for this pathogen, which was amplified by isothermal strand displacement amplification (SDA) and further detected by a lateral flow biosensor. As low as 10(1) colony forming unit (CFU) of S. enteritidis was detected in this study. Without DNA extraction, the reduced handling and simpler equipment requirement render this assay a simple and rapid alternative to conventional methods.

[1]  Maria Gazouli,et al.  Detection of Pathogenic Mycobacteria Based on Functionalized Quantum Dots Coupled with Immunomagnetic Separation , 2011, PloS one.

[2]  W. Prinyawiwatkul,et al.  Rapid and Specific Detection of Escherichia coli Serogroups O26, O45, O103, O111, O121, O145, and O157 in Ground Beef, Beef Trim, and Produce by Loop-Mediated Isothermal Amplification , 2012, Applied and Environmental Microbiology.

[3]  Ihab Abdel-Hamid,et al.  Detection of pathogenic bacteria in food samples using highly-dispersed carbon particles. , 2005, Biosensors & bioelectronics.

[4]  C. Hedberg,et al.  Food-related illness and death in the United States. , 1999, Emerging infectious diseases.

[5]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[6]  S. Manalis,et al.  Micromechanical detection of proteins using aptamer-based receptor molecules. , 2004, Analytical chemistry.

[7]  Guodong Liu,et al.  Lateral flow nucleic acid biosensor for Cu2+ detection in aqueous solution with high sensitivity and selectivity. , 2010, Chemical communications.

[8]  S. Huhulescu,et al.  An outbreak of febrile gastroenteritis associated with jellied pork contaminated with Listeria monocytogenes , 2009, Wiener klinische Wochenschrift.

[9]  D. Carminati,et al.  Effect of washing with a high pressure water spray on removal of Listeria innocua from Gorgonzola cheese rind , 2008 .

[10]  Reinhard Niessner,et al.  Detection of Escherichia coli O157:H7, Salmonella typhimurium, and Legionella pneumophila in water using a flow-through chemiluminescence microarray readout system. , 2008, Analytical chemistry.

[11]  Yanbin Li,et al.  Rapid, sensitive, and simultaneous detection of three foodborne pathogens using magnetic nanobead-based immunoseparation and quantum dot-based multiplex immunoassay. , 2011, Journal of food protection.

[12]  F Allerberger,et al.  Listeriosis: a resurgent foodborne infection. , 2010, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[13]  Reinhard Niessner,et al.  Sensitive quantification of Escherichia coli O157:H7, Salmonella enterica , and Campylobacter jejuni by combining stopped polymerase chain reaction with chemiluminescence flow-through DNA microarray analysis. , 2011, Analytical chemistry.

[14]  Nobuyasu Yamaguchi,et al.  Detection of Bacteria Carrying the stx2 Gene by In Situ Loop-Mediated Isothermal Amplification , 2003, Applied and Environmental Microbiology.

[15]  James R. Johnson,et al.  Diagnosis and treatment of acute pyelonephritis in women. , 2011, American family physician.

[16]  Feng Xu,et al.  Magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex PCR assay for simultaneous detection of viable Salmonella Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes in food products. , 2013, Food microbiology.

[17]  P K Surendran,et al.  Evaluation of culture, ELISA and PCR assays for the detection of Salmonella in seafood , 2007, Letters in applied microbiology.

[18]  Gia J. Oh,et al.  Characteristics of children with sporadic hemolytic uremic syndrome in a single Northern California center , 2012, International Urology and Nephrology.

[19]  R. Niessner,et al.  Immunomagnetic nanoparticle-based sandwich chemiluminescence-ELISA for the enrichment and quantification of E. coli , 2010 .

[20]  John Yew Huat Tang,et al.  Multiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium , 2011, Tropical medicine and health.

[21]  Soyoun Kim,et al.  Antibody-based biosensors for environmental monitoring , 2009, Toxicology and Environmental Health Sciences.

[22]  J. Frye,et al.  Sensitive and rapid molecular detection assays for Salmonella enterica serovars Typhimurium and Heidelberg. , 2009, Journal of food protection.

[23]  K. Mølbak,et al.  International Salmonella Typhimurium DT104 Infections, 1992–2001 , 2005, Emerging infectious diseases.

[24]  E. Alocilja,et al.  Market analysis of biosensors for food safety. , 2003, Biosensors & bioelectronics.

[25]  B. Jung,et al.  A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats , 2009, Journal of veterinary science.

[26]  D. Donoghue,et al.  Multiplex PCR assay for the detection and quantification of Campylobacter spp., Escherichia coli O157:H7, and Salmonella serotypes in water samples. , 2011, FEMS microbiology letters.

[27]  Rakesh Kumar,et al.  Rapid quantification of Salmonella in seafood by real-time PCR assay. , 2010, Journal of microbiology and biotechnology.

[28]  S. Salmaso,et al.  An outbreak of febrile gastroenteritis associated with corn contaminated by Listeria monocytogenes. , 2000, The New England journal of medicine.

[29]  Adrian McDonald,et al.  Quantification and control of microbial pollution from agriculture: a new policy challenge? , 2008 .

[30]  K L Josephson,et al.  Polymerase chain reaction detection of nonviable bacterial pathogens , 1993, Applied and environmental microbiology.

[31]  Yury E. Glazyrin,et al.  Development of bacteriostatic DNA aptamers for salmonella. , 2013, Journal of medicinal chemistry.

[32]  R. Yazdanparast,et al.  Development and evaluation of a loop-mediated isothermal amplification method in conjunction with an enzyme-linked immunosorbent assay for specific detection of Salmonella serogroup D. , 2012, Analytica chimica acta.

[33]  F. Ducongé,et al.  Aptamers against extracellular targets for in vivo applications. , 2005, Biochimie.

[34]  P. Sockett.  The economic implications of human Salmonella infection. , 1991, The Journal of applied bacteriology.

[35]  R. Yazdanparast,et al.  Development of a new loop-mediated isothermal amplification assay for prt (rfbS) gene to improve the identification of Salmonella serogroup D , 2012, World Journal of Microbiology and Biotechnology.

[36]  G. Pugazhenthi,et al.  Fabrication and properties of low cost ceramic microfiltration membranes for separation of oil and bacteria from its solution , 2011 .

[37]  R. Stoltenburg,et al.  SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands. , 2007, Biomolecular engineering.

[38]  J. Crump,et al.  Global trends in typhoid and paratyphoid Fever. , 2010, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.