Simultaneous production of acetate and methane from glycerol by selective enrichment of hydrogenotrophic methanogens in extreme-thermophilic (70°C) mixed culture fermentation

The feasibility of simultaneous production of acetate and methane from glycerol was investigated by selective enrichment of hydrogenotrophic methanogens in an extreme-thermophilic (70°C) fermentation. Fed-batch experiments showed acetate was produced at the concentration up to 13.0g/L. A stable operation of the continuous stirred tank reactor (CSTR) was reached within 100days. Acetate accounted for more than 90 w/w% of metabolites in the fermentation liquid. The yields of methane and acetate were close to the theoretical yields with 0.74–0.80mol-methane/mol-glycerol and 0.63–0.70mol-acetate/mol-glycerol. The obtained microbial community was characterized. Hydrogenotrophic methanogens, mainly Methanothermobacter thermautotrophicus formed 93% of the methanogenogenic community. This confirms that a high temperature (70°C) could effectively select for hydrogenotrophic methanogenic archaea. Thermoanaerobacter spp. was the main bacterium forming 91.5% of the bacterial population. This work demonstrated the conversion of the byproduct of biodiesel production, glycerol, to acetate as a chemical and biogas for energy generation.

[1]  M. V. van Loosdrecht,et al.  Mixed culture biotechnology for bioenergy production. , 2007, Current opinion in biotechnology.

[2]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[3]  Duu-Jong Lee,et al.  Microalgae-based biorefinery--from biofuels to natural products. , 2013, Bioresource technology.

[4]  V. S. Moholkar,et al.  Microbial conversion of glycerol: present status and future prospects , 2012, Critical reviews in biotechnology.

[5]  Shigeki Sawayama,et al.  Biodegradation and methane production from glycerol-containing synthetic wastes with fixed-bed bioreactor under mesophilic and thermophilic anaerobic conditions , 2008 .

[6]  F. A. Rainey,et al.  Purification and characterization of thermostable pectate-lyases from a newly isolated thermophilic bacterium, Thermoanaerobacter italicus sp. nov. , 1997, Extremophiles.

[7]  A. Stams,et al.  Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus. , 2003, Biotechnology and bioengineering.

[8]  Murray Moo-Young,et al.  Towards sustainable production of clean energy carriers from biomass resources , 2012 .

[9]  Ramon Gonzalez,et al.  Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals. , 2013, Trends in biotechnology.

[10]  Francesco Di Maria,et al.  Optimization of Solid State Anaerobic Digestion by inoculum recirculation: The case of an existing Mechanical Biological Treatment plant , 2012 .

[11]  Dalei Zhang,et al.  Biogas production and microcystin biodegradation in anaerobic digestion of blue algae , 2011 .

[12]  K. Finster,et al.  Biogeochemical and Molecular Signatures of Anaerobic Methane Oxidation in a Marine Sediment , 2001, Applied and Environmental Microbiology.

[13]  C. Howe,et al.  Biodiesel from algae: challenges and prospects. , 2010, Current opinion in biotechnology.

[14]  M. V. van Loosdrecht,et al.  Impact of oxygen limitation on glycerol-based biopolymer production by bacterial enrichments. , 2013, Water research.

[15]  T. Nunoura,et al.  Quantification of mcrA by fluorescent PCR in methanogenic and methanotrophic microbial communities. , 2008, FEMS microbiology ecology.

[16]  R. Gonzalez,et al.  Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. , 2007, Current opinion in biotechnology.

[17]  Yan Zhang,et al.  Hydrogen supersaturation in thermophilic mixed culture fermentation , 2012 .

[18]  S. Pavlostathis,et al.  Cellulose fermentation by continuous cultures of Ruminococcus albus and Methanobrevibacter smithii , 1990, Applied Microbiology and Biotechnology.

[19]  S. Schmidt,et al.  Anaerobic Digestion of Renewable Biomass: Thermophilic Temperature Governs Methanogen Population Dynamics , 2010, Applied and Environmental Microbiology.

[20]  Fang Zhang,et al.  Alkali production from bipolar membrane electrodialysis powered by microbial fuel cell and application for biogas upgrading , 2013 .

[21]  Robbert Kleerebezem,et al.  Glycerol fermentation by (open) mixed cultures: A chemostat study , 2008, Biotechnology and bioengineering.

[22]  Yan Zhang,et al.  Hydrogen supersaturation in extreme-thermophilic (70°C) mixed culture fermentation , 2013 .

[23]  G. K. Kafle,et al.  Anaerobic treatment of apple waste with swine manure for biogas production: Batch and continuous operation , 2013 .

[24]  B. Ahring,et al.  Effects of hydrogen and formate on the degradation of propionate and butyrate in thermophilic granules from an upflow anaerobic sludge blanket reactor , 1993, Applied and environmental microbiology.

[25]  Stefan Schmidt,et al.  The microcosm of a biogas fermenter: Comparison of moderate hyperthermophilic (60°C) with thermophilic (55°C) conditions , 2010 .

[26]  Anne-Kristin Kaster,et al.  Methanogenic archaea: ecologically relevant differences in energy conservation , 2008, Nature Reviews Microbiology.

[27]  Wahyudiono,et al.  Selective Conversion of Glucose into Lactic Acid and Acetic Acid with Copper Oxide Under Hydrothermal Conditions , 2013 .

[28]  B. Ahring,et al.  Thermoanaerobacter mathranii sp. nov., an ethanol-producing, extremely thermophilic anaerobic bacterium from a hot spring in Iceland , 1997, Archives of Microbiology.

[29]  J. G. Kuenen,et al.  Biochemical limits to microbial growth yields: An analysis of mixed substrate utilization , 1988, Biotechnology and bioengineering.

[30]  A. Obraztsova,et al.  Methanobacterium thermoflexum sp. nov. and Methanobacterium defluvii sp. nov., Thermophilic Rod-Shaped Methanogens Isolated from Anaerobic Digestor Sludge , 1993 .

[31]  Damien J. Batstone,et al.  Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge , 2013, Applied and Environmental Microbiology.

[32]  C. Carrington,et al.  Variables affecting the in situ transesterification of microalgae lipids , 2010 .

[33]  M. V. van Loosdrecht,et al.  Stable acetate production in extreme-thermophilic (70°C) mixed culture fermentation by selective enrichment of hydrogenotrophic methanogens , 2014, Scientific Reports.

[34]  M. V. van Loosdrecht,et al.  A modified metabolic model for mixed culture fermentation with energy conserving electron bifurcation reaction and metabolite transport energy , 2013, Biotechnology and bioengineering.

[35]  J. Reeve,et al.  Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanot , 2000, International journal of systematic and evolutionary microbiology.

[36]  Yanhe Ma,et al.  Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, China. , 2001, International journal of systematic and evolutionary microbiology.

[37]  A. Rodrigues,et al.  Glycerol Reforming for Hydrogen Production: A Review , 2009 .

[38]  Jinyue Yan,et al.  Biofuels in Asia , 2009 .

[39]  S. Olsen,et al.  A critical review of biochemical conversion, sustainability and life cycle assessment of algal biofuels , 2011 .

[40]  Yan Zhang,et al.  Fatty acids production from hydrogen and carbon dioxide by mixed culture in the membrane biofilm reactor. , 2013, Water research.