Sequestration of Stigmasterol and β-Sitosterol from Ethanolic Extract of Kam Sabut (Croton caudatus Geiseler) Leaves

Croton caudatus (Kam sabut) has been traditionally used to treat cancer in northeastern India and earlier studies reported the anticancer activity of ethanolic extract of C. caudatus leaves in HeLa cells in vitro and Swiss albino mice transplanted with Dalton’s ascites lymphoma. Therefore, isolation of stigmasterol and β-sitosterol from the ethanolic extract of C. caudatus Geiseler leaves was carried out. Stigmasterol and β-sitosterol were isolated from the ethanolic extract of dried leaves of C. caudatus by column chromatography. The fractions were purified further on Sephadex® LH-20 column chromatography and reverse-phase HPLC. The isolated phytosterols were characterized and quantified by IR, 1H & 13 CNMR, mass spectroscopy and HPLC. This has led to the separation of 100 mg stigmasterol and 80 mg β-sitosterol. Stigmasterol and β-sitosterol have been isolated from the ethanolic extract of C. caudatus leaves for the first time.

[1]  David J Newman,et al.  Natural Products as Sources of New Drugs over the Nearly Four Decades from 01/1981 to 09/2019. , 2020, Journal of natural products.

[2]  G. Jagetia,et al.  Anticancer activity of an ehnomedicinal plant Croton caudatus Geiseler, Kam sabut in cultured HeLa cells , 2020 .

[3]  Gerard D. Wright.  Unlocking the potential of natural products in drug discovery , 2018, Microbial biotechnology.

[4]  K. Gwoździński,et al.  Anticancer Activity of Natural Compounds from Plant and Marine Environment , 2018, International journal of molecular sciences.

[5]  F. Visioli,et al.  Effects of extracellular acidity on resistance to chemotherapy treatment: a systematic review , 2018, Medical Oncology.

[6]  H. Kim,et al.  Chemoresistance of Cancer Cells: Requirements of Tumor Microenvironment-mimicking In Vitro Models in Anti-Cancer Drug Development , 2018, Theranostics.

[7]  Md. Eaqub Ali,et al.  Techniques for the extraction of phytosterols and their benefits in human health: a review , 2018 .

[8]  N. Staff,et al.  Chemotherapy‐induced peripheral neuropathy: A current review , 2017, Annals of neurology.

[9]  Petra Schneider,et al.  Counting on natural products for drug design. , 2016, Nature chemistry.

[10]  M. Kinch,et al.  An analysis of FDA-approved drugs: natural products and their derivatives. , 2016, Drug discovery today.

[11]  J. Pezzuto,et al.  Natural Products as a Vital Source for the Discovery of Cancer Chemotherapeutic and Chemopreventive Agents , 2015, Medical Principles and Practice.

[12]  G. P. Dubey,et al.  Identification of beta-sitosterol and stigmasterol in Bambusa bambos (L.) Voss leaf extract using HPLC and its estrogenic effect in vitro. , 2015, Journal of pharmaceutical and biomedical analysis.

[13]  Michael A Fischbach,et al.  Computational approaches to natural product discovery. , 2015, Nature chemical biology.

[14]  A. Harvey,et al.  The re-emergence of natural products for drug discovery in the genomics era , 2015, Nature Reviews Drug Discovery.

[15]  I. Prakash,et al.  Isolation of Stigmasterol and β-Sitosterol from the dichloromethane extract of Rubus suavissimus , 2012 .

[16]  G. Seru,et al.  Phytochemical and pharmacological evaluation of Tragia cannabina for anti-inflammatory activity , 2012 .

[17]  C. S. Reddy,et al.  In-vitro and in-vivo anti-inflammatory activity of Andrographis serpyllifolia (Rottl. Ex Vahl.) Wt. , 2012 .

[18]  K. Moxley,et al.  Endometrial Carcinoma: A Review of Chemotherapy, Drug Resistance, and the Search for New Agents , 2010, The oncologist.

[19]  M. Rahman,et al.  Isolation of stigmasterol and beta-sitosterol from methanolic extract of root bark of Calotropis gigantea (Linn). , 2007, Pakistan journal of biological sciences : PJBS.