Ginsenoside production by hairy root cultures of Panax ginseng: influence of temperature and light quality

Abstract Large-scale production of valuable secondary metabolites by plant cell cultures is generally hampered by low productivity. This productivity is affected by several physical factors like temperature and light. In this study we have investigated the impact of temperature and light quality on biomass accumulation and ginsenoside production by hairy roots cultivated in large-scale bioreactors. Biomass accumulation and ginsenoside production was optimal under 20 °C/13 °C day (12 h)/night (8 h) cycle. Biomass of hairy roots was highest in the cultures grown under dark or red light while ginsenoside accumulation was optimum in the cultures grown under fluorescent light.

[1]  Noboru Hiraoka,et al.  Pigment formation in callus cultures of Lithospermum erythrorhizon , 1974 .

[2]  J. W. Heo,et al.  Induction of Betalain Pigmentation in Hairy Roots of Red Beet under Different Radiation Sources , 2003, Biologia Plantarum.

[3]  T. Furuya,et al.  Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes , 1987, Plant Cell Reports.

[4]  R. E. Kendrick,et al.  PHOTOMORPHOGENESIS IN PLANTS , 1990 .

[5]  R. Verpoorte,et al.  Production of pulchelin E in hairy roots, callus and suspension cultures of Rudbeckia hirta L. , 2002 .

[6]  M. Bonfill,et al.  Elicitation of different Panax ginseng transformed root phenotypes for an improved ginsenoside production , 2003 .

[7]  J. Tramper,et al.  Stratégies to improve the production of secondary metabolites with plant cell cultures : a literature review , 1992 .

[8]  Chen Guo,et al.  Effect of light irradiation on hairy root growth and artemisinin biosynthesis of Artemisia annua L , 2002 .

[9]  Professor Dr. Weici Tang,et al.  Chinese Drugs of Plant Origin , 1992, Springer Berlin Heidelberg.

[10]  Seki,et al.  Intermittent light irradiation with second- or hour-scale periods controls anthocyanin production by strawberry cells* , 2000, Enzyme and microbial technology.

[11]  R. U. Schenk,et al.  Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures , 1972 .

[12]  Toshiomi Yoshida,et al.  Effect of light irradiation on anthocyanin production by suspended culture of Perilla frutescens , 1991, Biotechnology and bioengineering.

[13]  Wenyuan Gao,et al.  Improvement of ginsenoside production by jasmonic acid and some other elicitors in hairy root culture of ginseng (Panax ginseng C. A. Meyer) , 2000, In Vitro Cellular & Developmental Biology - Plant.

[14]  S. M. Choi,et al.  Induction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng , 1999 .

[15]  Jian-Jiang Zhong,et al.  Effects of temperature on cell growth and anthocyanin production in suspension cultures of Perilla frutescens , 1993 .

[16]  J. Zhong,et al.  Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects. , 1999, Journal of biotechnology.

[17]  Y. Gozu,et al.  Growth pattern and ginsenoside production of Agrobacterium-transformed Panax ginseng roots , 1993, Plant Cell Reports.

[18]  F. Kreuzaler,et al.  Flavonoid glycosides from illuminated cell suspension cultures of Petroselinum hortense , 1973 .

[19]  J. J. Heijnen,et al.  Influence of temperature on growth and ajmalicine production by Catharantus roseus suspension cultures , 2002 .