Operator-assisted harvesting of protein crystals using a universal micromanipulation robot

The prototype of a universal micromanipulation robot for crystal harvesting is presented, and a robotically harvested trypsin crystal yields a high-resolution structure demonstrating the feasibility of robotic protein crystal harvesting.

[1]  R. Thorne,et al.  Hyperquenching for protein cryocrystallography. , 2006, Journal of applied crystallography.

[2]  A. McPherson,et al.  Searching for silver bullets: an alternative strategy for crystallizing macromolecules. , 2006, Journal of structural biology.

[3]  J. Thornton,et al.  PROCHECK: a program to check the stereochemical quality of protein structures , 1993 .

[4]  E A Merritt,et al.  Raster3D: photorealistic molecular graphics. , 1997, Methods in enzymology.

[5]  Tom L. Blundell,et al.  Keynote review: Structural biology and drug discovery , 2005 .

[6]  Christian X Weichenberger,et al.  Self-consistent assignment of asparagine and glutamine amide rotamers in protein crystal structures. , 2006, Structure.

[7]  J. Pflugrath,et al.  Macromolecular cryocrystallography--methods for cooling and mounting protein crystals at cryogenic temperatures. , 2004, Methods.

[8]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[9]  Andrea Schmidt,et al.  Trypsin Revisited , 2003, Journal of Biological Chemistry.

[10]  C. Sander,et al.  Errors in protein structures , 1996, Nature.

[11]  D E McRee,et al.  XtalView/Xfit--A versatile program for manipulating atomic coordinates and electron density. , 1999, Journal of structural biology.

[12]  Bernhard Rupp.  High-throughput crystallography at an affordable cost: the TB Structural Genomics Consortium Crystallization Facility. , 2003, Accounts of chemical research.

[13]  良二 上田.  J. Appl. Cryst.の発刊に際して , 1970 .

[14]  J Otlewski,et al.  The crystal structures of the complexes between bovine beta-trypsin and ten P1 variants of BPTI. , 2000, Journal of molecular biology.

[15]  Elspeth F Garman,et al.  Towards an understanding of radiation damage in cryocooled macromolecular crystals. , 2005, Journal of synchrotron radiation.

[16]  Robert E. Thorne,et al.  Microfabricated mounts for high-throughput macromolecular cryocrystallography , 2003 .

[17]  R. Huber,et al.  Accurate Bond and Angle Parameters for X-ray Protein Structure Refinement , 1991 .

[18]  E. Dodson,et al.  Phenol stabilizes more helix in a new symmetrical zinc insulin hexamer , 1989, Nature.

[19]  Daniel Scheibe,et al.  A fully integrated protein crystallization platform for small-molecule drug discovery. , 2003, Journal of structural biology.

[20]  Ruben Abagyan,et al.  ICM—A new method for protein modeling and design: Applications to docking and structure prediction from the distorted native conformation , 1994, J. Comput. Chem..

[21]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.