Cu2+ Detection with Gold Nanoparticles by Patterning Colorimetric Strips on a Filter Membrane Assembled in a Microfluidic Chip

We have developed a microfluidic chip for colorimetric Cu2+ detection. In this chip, it is facile to do colorimetric Cu2+ detection based on gold nanoparticles. This method has a dynamic detection range from 0.75 to 50 µmol/L with only 20 µL solution including detection reagents and sample. The result can be readout by naked eye and photographed by digital cameras. With the help of image processing software, we could measure the RGB value and calculate the Blue/Red ratio for more accurate quantification. Tap water could be detected in this portable chip.

[1]  Jiahui Yu,et al.  Enhanced Sensitivity and Selectivity of Chemosensor for Malonate by Anchoring on Gold Nanoparticles , 2011 .

[2]  Qing X. Li,et al.  Detection of copper ions using microcantilever immunosensors and enzyme-linked immunosorbent assay. , 2010, Analytica chimica acta.

[3]  Wei Zhang,et al.  Highly sensitive, colorimetric detection of mercury(II) in aqueous media by quaternary ammonium group-capped gold nanoparticles at room temperature. , 2010, Analytical chemistry.

[4]  Chunhai Fan,et al.  Design of a gold nanoprobe for rapid and portable mercury detection with the naked eye. , 2008, Chemical communications.

[5]  Kyung-Jin Jang,et al.  Detection of proteins using a colorimetric bio-barcode assay , 2007, Nature Protocols.

[6]  Yi Lu,et al.  Abasic site-containing DNAzyme and aptamer for label-free fluorescent detection of Pb(2+) and adenosine with high sensitivity, selectivity, and tunable dynamic range. , 2009, Journal of the American Chemical Society.

[7]  M. Masár,et al.  Determination of ammonium, calcium, magnesium, potassium and sodium in drinking waters by capillary zone electrophoresis on a column-coupling chip. , 2009, Journal of chromatography. A.

[8]  Xingyu Jiang,et al.  Copper-mediated amplification allows readout of immunoassays by the naked eye. , 2011, Angewandte Chemie.

[9]  George M Whitesides,et al.  What comes next? , 2011, Lab on a chip.

[10]  G. Shen,et al.  Colorimetric Sensing of Adenosine Based on Aptamer Binding Inducing Gold Nanoparticle Aggregation , 2009 .

[11]  Chih-Ching Huang,et al.  Gold nanoparticle-based colorimetric assays for coagulation-related proteins and their inhibition reactions. , 2011, Biosensors & bioelectronics.

[12]  M. Zhang,et al.  Colorimetric assay for sulfate using positively-charged gold nanoparticles and its application for real-time monitoring of redox process. , 2011, The Analyst.

[13]  Chad A Mirkin,et al.  Colorimetric detection of mercuric ion (Hg2+) in aqueous media using DNA-functionalized gold nanoparticles. , 2007, Angewandte Chemie.

[14]  Xingyu Jiang,et al.  Visual detection of copper(II) by azide- and alkyne-functionalized gold nanoparticles using click chemistry. , 2008, Angewandte Chemie.

[15]  Erkang Wang,et al.  Microfabricated on-chip integrated Au-Ag-Au three-electrode system for in situ mercury ion determination. , 2010, The Analyst.

[16]  Andrew J. deMello,et al.  Surface-enhanced Raman scattering in nanoliter droplets: towards high-sensitivity detection of mercury (II) ions , 2009, Analytical and bioanalytical chemistry.

[17]  Hongkai Wu,et al.  Fabrication of a microfluidic Ag/AgCl reference electrode and its application for portable and disposable electrochemical microchips , 2010, Electrophoresis.

[18]  Xingyu Jiang,et al.  Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles. , 2011, Biosensors & bioelectronics.