A Medipix quantum area detector allows rotation electron diffraction data collection from submicrometre three-dimensional protein crystals

An ultrasensitive Medipix2 detector allowed the collection of rotation electron-diffraction data from single three-dimensional protein nanocrystals for the first time. The data could be analysed using the standard X-ray crystallography programs MOSFLM and SCALA.

[1]  J. Abrahams,et al.  Evaluation of Medipix2 detector for recording electron diffraction data in low dose conditions , 2011 .

[2]  J. Abrahams The strong phase object approximation may allow extending crystallographic phases of dynamical electron diffraction patterns of 3D protein nano-crystals , 2010 .

[3]  R. Henderson,et al.  Images of purple membrane at 2.8 A resolution obtained by cryo-electron microscopy. , 1988, Journal of molecular biology.

[4]  Daniel Turecek,et al.  Pixelman: a multi-platform data acquisition and processing software package for Medipix2, Timepix and Medipix3 detectors , 2011 .

[5]  J. Koenderink Q… , 2014, Les noms officiels des communes de Wallonie, de Bruxelles-Capitale et de la communaute germanophone.

[6]  R. Henderson The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules , 1995, Quarterly Reviews of Biophysics.

[7]  R. Dinapoli,et al.  Medipix2: A 64-k pixel readout chip with 55-/spl mu/m square elements working in single photon counting mode , 2001 .

[8]  H. Schenk,et al.  MSLS, a Least-Squares Procedure for Accurate Crystal Structure Refinement from Dynamical Electron Diffraction Patterns , 1998 .

[9]  R. Henderson,et al.  Electron imaging with Medipix2 hybrid pixel detector. , 2007, Ultramicroscopy.

[10]  N. Chayen Protein crystallization strategies for structural genomics , 2007 .

[11]  P. Evans,et al.  Scaling and assessment of data quality. , 2006, Acta crystallographica. Section D, Biological crystallography.

[12]  David San Segundo Bello,et al.  An interface board for the control and data acquisition of the Medipix2 chip , 2003 .

[13]  R. Rosenfeld Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[14]  Randy J. Read,et al.  Evolving Methods for Macromolecular Crystallography , 2007 .

[15]  J. Abrahams,et al.  Unit-cell determination from randomly oriented electron-diffraction patterns , 2009, Acta crystallographica. Section D, Biological crystallography.

[16]  Xing Zhang,et al.  3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry , 2010, Cell.

[17]  U. Kolb,et al.  Automated electron diffraction tomography – a new tool for nano crystal structure analysis , 2011 .

[18]  R Henderson,et al.  Electronic detectors for electron microscopy. , 2007, Current opinion in structural biology.

[19]  R. Henderson,et al.  Detective quantum efficiency of electron area detectors in electron microscopy , 2009, Ultramicroscopy.

[20]  Georg Weidenspointner,et al.  Femtosecond X-ray protein nanocrystallography , 2011, Nature.

[21]  Zdenek Vykydal,et al.  USB interface for Medipix2 pixel device enabling energy and position-sensitive detection of heavy charged particles , 2006 .

[22]  B. van der Heijden,et al.  A Gigabit per second read-out system for Medipix Quads , 2011 .