Development of reverse-transcription loop-mediated isothermal amplification assay (RT-LAMP) for the detection of Barley yellow mosaic virus

Summary Barley yellow mosaic virus (BaYMV) is the causal agent of a dangerous disease of winter barley, identified in Poland for the first time in 2008. It is transmitted by a root-inhabiting fungal-like plasmodiophorid Polymyxa graminis Led, and thus the disease is called “soil-borne”. Taking into account the way of transmission of the pathogen, the only method of protection of winter barley crops against this pathogen is breeding and cultivation of resistant/tolerant cultivars. The objective of investigations presented in this paper was to develop an improved efficient diagnostic molecular method for precise detection of BaYMV in assessed materials. For the first time a reverse-transcription loop-mediated isothermal amplification assay (RT-LAMP) was chosen and adopted for improved diagnostics of BaYMV. The specific RT-LAMP primers for BaYMV detection were designed based on the coat protein gene. RT-LAMP assay is inexpensive and easy to perform, so it may be used for the rapid and specific diagnosis of BaYMV.

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