Bone Formation Induced by Recombinant Human BMP-2 Combined with Bioabsorbable Material at Subperiosteal Sites

SYNOPSIS The purpose of this study was to evaluate the effect of bone formation induced by recombinant human BMP-2 (rhBMP-2) combined with bioabsorbable material KurabioAG (AG) at palatal subperiosteal sites in Wistar rats. AG was freeze-dried alginated gel dressing. Experimental sites were divided into three groups according to the implans : BMP group (rhBMP-2 combined with AG), AG group (AG alone) and Control group. In every group, infected region or atypical cell proliferation was not observed, and new bone formation was observed at 6 weeks after implantation. Thickness of new bone (TNB) of BMP group was significantly higher than that of AG and Control groups. There was no significant difference in TNB between AG and Control groups. These results suggest that rhBMP-2 combined with AG have the ability to promote bone formation, although AG alone do not have any effect on bone formation.

[1]  K. Yamaji,et al.  Study on Bone Formation Induced by Recombinant Human BMP-2 at Palatal Sites in Adult Rats , 2006 .

[2]  Y. Komasa,et al.  Dentin regeneration by direct pulp capping using a bioabsorbable material , 2006 .

[3]  L. Cheung,et al.  Effect of Recombinant Human Bone Morphogenetic Protein-2 on Mandibular Distraction at Different Rates in an Experimental Model , 2006, The Journal of craniofacial surgery.

[4]  Seung Jin Lee,et al.  Immobilization of bone morphogenetic protein‐2 on a nanofibrous chitosan membrane for enhanced guided bone regeneration , 2006, Biotechnology and applied biochemistry.

[5]  R. Welch,et al.  Recombinant Human Bone Morphogenic Protein-2-Enhanced Anterior Spine Fusion Without Bone Encroachment Into the Spinal Canal: A Histomorphometric Study in a Thoracoscopically Instrumented Porcine Model , 2005, Spine.

[6]  B. Feeley,et al.  Lentivirus-mediated gene transfer induces long-term transgene expression of BMP-2 in vitro and new bone formation in vivo. , 2005, Molecular therapy : the journal of the American Society of Gene Therapy.

[7]  D. Aizenbud,et al.  Enhancement of bone formation by bone morphogenetic protein-2 during alveolar distraction: an experimental study in sheep. , 2004, Journal of periodontology.

[8]  Johnny Huard,et al.  Enhancement of bone healing based on ex vivo gene therapy using human muscle-derived cells expressing bone morphogenetic protein 2. , 2002, Human gene therapy.

[9]  M. Kawanami,et al.  Effect of aging on bone formation induced by recombinant human bone morphogenetic protein-2 combined with fibrous collagen membranes at subperiosteal sites. , 2001, Journal of periodontal research.

[10]  熈 加藤,et al.  リコンビナントヒトBMP-2をポリ乳酸グリコール酸共重合体/ゼラチンスポンジ複合体に配合してラット口蓋部骨膜下に移植した場合の骨形成に対する加齢の影響 , 1999 .

[11]  J. Wozney,et al.  Periodontal repair in dogs: effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment. , 1999, Journal of clinical periodontology.

[12]  Y. Shimizu,et al.  A Novel Wound Dressing With an Antibiotic Delivery System Stimulated by Microbial Infection , 1997, ASAIO journal.

[13]  V. Rosen,et al.  The effects of aging on the bone inductive activity of recombinant human bone morphogenetic protein-2. , 1996, Endocrinology.

[14]  V. Rosen,et al.  Recombinant human bone morphogenetic protein induces bone formation. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[15]  B. Feeley,et al.  Lentivirus-mediated gene transfer induces long-term transgene expression of BMP-2 in vitro and new bone formation in vivo. , 2005, Molecular Therapy.

[16]  M C Sogayar,et al.  Bone morphogenetic proteins: from structure to clinical use. , 2005, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.