论文信息 - STUDIES ON THE PROTEOLYTIC ENZYMES OF A COMMERCIAL MALT

STUDIES ON THE PROTEOLYTIC ENZYMES OF A COMMERCIAL MALT

Two proteolytic enzymes, Hb-ase and BAPA-ase, were partially purified from commercial malt. Hb-ase was inhibited by bromate, N-ethylmaleimide and p-chloromercuriphenylsulphonic acid and activated by ethylenediaminetetra-acetic acid. BAPA-ase was inhibited by N-ethylmaleimide and p-hydroxychloromercuribenzoate but not by bromate or phenylmethylsulphonylfluoride, a specific trypsin inhibitor. The results obtained suggested that BAPA-ase, like the Hb-ase, is a thiol enzyme.

R. S. Bhatty

[1] A. Gold,et al. Sulfonyl Fluorides as Inhibitors of Esterases. I. Rates of Reaction with Acetylcholinesterase, α-Chymotrypsin, and Trypsin , 1963 .

[2] William W. Cohen,et al. The preparation and properties of two new chromogenic substrates of trypsin. , 1961, Archives of biochemistry and biophysics.

[3] W. Hughes,et al. [10] Protein fractionation on the basis of solubility in aqueous solutions of salts and organic solvents , 1955 .