In vitro effects of hyperthermia combined with cisplatin or peplomycin on the human maxillary carcinoma cell line IMC-2.

We examined the interactive effects of hyperthermia combined with cisplatin (CDDP) (0.5 micrograms/ml) or peplomycin (PEP) (1.0 microgram/ml) on surviving fractions of human maxillary carcinoma IMC-2 cells. Either CDDP or PEP enhanced the 44 degree C thermosensitivity of thermotolerant cells after heating at 42 degrees C for 2 hours. The development of thermotolerance at 42 degrees C with either of the two drugs for 2 hours was not inhibited by CDDP, but it was partially inhibited by PEP. Moreover, for PEP throughout the entire period of 42-44 degrees C step-up heating, the 44 degree C thermosensitivity of thermotolerant cells after heating at 42 degrees C with PEP for 2 hours was enhanced similarly to that at 44 degrees C with PEP. Heating at 42 degrees C combined with either of the two drugs showed a marked interactive effect.

[1]  T. Ohtsubo,et al.  Effect of heat-drug sequences on thermoenhancement and uptake of cis-DDP in human pharyngeal carcinoma. , 1996, Anticancer research.

[2]  T. Kaneda,et al.  Effect of ATP on the release of hsp 70 and hsp 40 from the nucleus in heat-shocked HeLa cells. , 1993, Experimental cell research.

[3]  G. Li,et al.  Expression of human hsp70 in rat fibroblasts enhances cell survival and facilitates recovery from translational and transcriptional inhibition following heat shock. , 1992, Cancer research.

[4]  G. Li,et al.  Thermal response of rat fibroblasts stably transfected with the human 70-kDa heat shock protein-encoding gene. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[5]  T. Ohtsubo,et al.  Effects of cis-diamminedichloroplatinum (CDDP) and cis-diammine (1,1-cyclobutanedicarboxylate) platinum (CBDCA) on thermotolerance development and thermosensitivity of the thermotolerant cells. , 1990, International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group.

[6]  J. Landry,et al.  Heat shock resistance conferred by expression of the human HSP27 gene in rodent cells , 1989, The Journal of cell biology.

[7]  Y. Kuratomi,et al.  Establishment of tumor cell lines from a patient with head and neck cancer and their different sensitivities to anti‐cancer agents , 1989, Cancer.

[8]  T. Kondo,et al.  Sensitivities of bleomycin-resistant variant cells enhanced by 40C hyperthermia in vitro , 1988 .

[9]  E. Gerner,et al.  Heat shock proteins in thermotolerance and other cellular processes. , 1987, Cancer research.

[10]  R. Engelhardt.  Hyperthermia and drugs. , 1987, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer.

[11]  K. Henle,et al.  Cytotoxicity of hyperthermia combined with bleomycin or cis-platinum in cultured RIF cells: modification by thermotolerance and by polyhydroxy compounds. , 1986, Cancer research.

[12]  D. Lydall,et al.  Mechanism of cytotoxicity of anticancer platinum drugs: evidence that cis-diamminedichloroplatinum(II) and cis-diammine-(1,1-cyclobutanedicarboxylato)platinum(II) differ only in the kinetics of their interaction with DNA. , 1986, Cancer research.

[13]  T. Samulski,et al.  Anatomical site-specific modalities for hyperthermia. , 1984, Cancer research.

[14]  G. Bartosz,et al.  Hyperthermic modification of bleomycin--DNA interaction detected by electron spin resonance. , 1983, Radiation research.

[15]  F. Friedlos,et al.  The frequency of interstrand cross-links in DNA following reaction of cis-diamminedichloroplatinum(II) with cells in culture or DNA in vitro: stability of DNA cross-links and their repair. , 1982, Chemico-biological interactions.

[16]  R. Meyn,et al.  Thermal enhancement of DNA damage in mammalian cells treated with cis-diamminedichloroplatinum(II). , 1980, Cancer research.

[17]  R. Meyn,et al.  Thermal enhancement of DNA strand breakage in mammalian cells treated with bleomycin. , 1979, International journal of radiation oncology, biology, physics.

[18]  E. Gerner,et al.  Induced thermal resistance in HeLa cells , 1975, Nature.