Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide

Abstract The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, 25°C culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of 107 conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.

[1]  Ashok Pandey,et al.  Solid-state fermentation , 1994 .

[2]  J. V. Alphen,et al.  Interactions among the entomopathogenic fungus, Beauveria bassiana (Ascomycota: Hypocreales), the parasitoid, Aphidius matricariae (Hymenoptera: Braconidae), and its host, Myzus persicae (Homoptera: Aphididae) , 2009 .

[3]  Seon-Gon Kim,et al.  Production of Blastospore of Entomopathogenic Beauveria bassiana in a Submerged Batch Culture , 2009, Mycobiology.

[4]  H. Sharma.  Pest Management and the Environment , 2008 .

[5]  H. Sharma.  Biotechnological Approaches for Pest Management and Ecological Sustainability , 2008 .

[6]  Keun Kim,et al.  Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide , 2008, Mycobiology.

[7]  F. Posada-Flórez.  Production of Beauveria bassiana fungal spores on rice to control the coffee berry borer, Hypothenemus hampei, in Colombia , 2008 .

[8]  Bin Chen,et al.  Occurrence of entomopathogenic fungi in migratory alate aphids in Yunnan Province of China , 2008, BioControl.

[9]  M. Rombach.  Production ofbeauveria bassiana [Deuteromycotina. Hyphomycetes] sympoduloconidia in submerged culture , 1989, Entomophaga.

[10]  S. Ying,et al.  Trials of Beauveria bassiana, Paecilomyces fumosoroseus and imidacloprid for management of Trialeurodes vaporariorum (Homoptera: Aleyrodidae) on greenhouse grown lettuce , 2004 .

[11]  Z. Baysal,et al.  Solid state fermentation for production of α-amylase by a thermotolerant Bacillus subtilis from hot-spring water , 2003 .

[12]  D. L. Johnson,et al.  Biological control of locusts and grasshoppers. , 2001, Annual review of entomology.

[13]  Bing-Lan Liu,et al.  Verticillium lecanii spore production in solid-state and liquid-state fermentations , 2000 .

[14]  M. Raimbault,et al.  General and microbiological aspects of solid substrate fermentation , 1998 .

[15]  J. Langewald,et al.  Development of mass production technology for aerial conidia for use as mycopesticides , 1998 .

[16]  M. Thomas,et al.  Effects of temperature on growth of Metarhizium flavoviride and virulence to the variegated grasshopper, Zonocerus variegatus , 1997 .

[17]  T. Glare,et al.  LARGE SCALE PRODUCTION OF NEW ZEALAND STRAINS OF BEAUVERIA AND METARHIZIUM , 1996 .

[18]  T. Satyanarayana,et al.  α-Amylase production by thermophilic Bacillus coagulans in solid state fermentation , 1995 .

[19]  George G. Khachatourians,et al.  Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status. , 1994 .

[20]  R. S. St. Leger,et al.  INTERACTIONS BETWEEN FUNGAL PATHOGENS AND INSECT HOSTS , 1994 .

[21]  Y. Ibrahim,et al.  Potential of mass‐production and field efficacy of isolates of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus against Plutella xylostella , 1993 .

[22]  G. Zimmermann,et al.  Production of blastospores by three strains of metarhizium anisopliae (metch.) sorokin in submerged culture , 1992 .

[23]  E. Aregger.  Conidia production of the fungus Beauveria brongniartii on barley and quality evaluation during storage at 2°C , 1992 .

[24]  S. Hill.  Pest control in sustainable agriculture. , 1990 .

[25]  M. Burge.  Fungi in biological control systems , 1989 .

[26]  R. Bosch,et al.  Impact of Pathogens, Parasites, and Predators on Aphids , 1968 .