Successful engraftment of cryopreserved autologous bone marrow in patients with malignant lymphoma

In an effort to evaluate the possible utility of cryopreserved autologous bone marrow infusions in man, 22 patients with malignant lymphoma resistant to conventional chemotherapy were treated with high- dose chemotherapy. This was followed in 12 patients by an infusion of their cryopreserved autologous bone marrow; 10 patients received chemotherapy alone and serve as controls. Following chemotherapy, severe leukopenia (less than 100 leukocytes/mm3) lasted 6–10 (median 8) days in patients receiving cryopreserved marrow, compared to 10–29 (median 16) days in controls (p less than 0.001). Recovery to 1000 leukocytes/mm3 occurred 10–18 (median 13) days after chemotherapy in autograft recipients but was delayed until 12–38 (median 23) days after chemotherapy in controls (p less than 0.001). Autografted patients also recovered granulocyte and platelet function significantly faster and had significantly fewer febrile days after chemotherapy than did controls. Cryopreserved autologous bone marrow infusions can hasten hemopoietic recovery in man after high-dose chemotherapy; this earlier reconstitution may be of clinical benefit to the patient.

[1]  F. Appelbaum,et al.  Prolonged complete remission following high dose chemotherapy of burkitt's lymphoma in relapse , 1978, Cancer.

[2]  R. Weiner,et al.  Cryopreserved autologous marrow infusion following high dose cancer chemotherapy. , 1977, European journal of cancer.

[3]  V. Devita,et al.  Combined modality treatment of American Burkittapos;s lymphoma , 1976 .

[4]  R. Weiner,et al.  Increase in Circulating Stem Cells Following Chemotherapy in Man , 1976 .

[5]  V. Ferrans,et al.  ACUTE LETHAL CARDITIS CAUSED BY HIGH-DOSE COMBINATION CHEMOTHERAPY A Unique Clinical and Pathological Entity , 1976, The Lancet.

[6]  M. Tattersall,et al.  Perspectives in cancer research. Autologous Marrow Support and intensive chemotherapy in cancer patients. , 1976, European journal of cancer.

[7]  J. Bull,et al.  Bone-marrow transplantation following combination chemotherapy immunosuppression (B.A.C.T.) in patients with acute leukemia. , 1974, Transplantation proceedings.

[8]  A. Ragab,et al.  The cryopreservation of colony-forming cells from the bone marrow of children with acute-lymphocytic leukemia. , 1974, Cancer research.

[9]  Young Rc,et al.  Hematologic malignancies and other marrow failure states: progress in the management of complicating infections. , 1974 .

[10]  P. Neiman,et al.  Treatment of blastic transformation of chronic granulocytic leukemia by high dose cyclophosphamide, total body irradiation and infusion of cryopreserved autologous marrow. , 1974, Experimental hematology.

[11]  W. Robinson,et al.  In vitro colony formation by human bone marrow cells after freezing. , 1973, The Journal of laboratory and clinical medicine.

[12]  J. Adamson,et al.  THE PROLIFERATIVE POTENTIAL OF FROZEN STORED HUMAN MARROW CELLS , 1972, Transplantation.

[13]  R Storb,et al.  Technique for human marrow grafting. , 1970, Blood.

[14]  R. Storb,et al.  Low temperature preservation of monkey marrow in dimethyl sulfoxide. , 1970, Cryobiology.

[15]  A. Goldin.  Factors pertaining to complete drug-induced remission of tumor in animals and man. , 1969, Cancer research.

[16]  Y. S. Sathe,et al.  Quantitative relationships between circulating leukocytes and infection in patients with acute leukemia. , 1966, Annals of internal medicine.

[17]  E. Thomas,et al.  Recovery of lethally irradiated dogs following infusion of autologous marrow stored at low temperature in dimethylsulphoxide. , 1962, Blood.

[18]  N. Kurnick.  Autologous and Isologous Bone Marrow Storage and Infusion in the Treatment of Myelo‐Suppression * , 1962, Transfusion.

[19]  H. Skipper,et al.  A manual on quantitative drug evaluation in experimental tumor systems. I. Background, description of criteria, and presentation of quantitative therapeutic data on various classes of drugs obtained in diverse experimental tumor systems. , 1962, Cancer chemotherapy reports.

[20]  A. Goldin,et al.  A manual on quantitative drug evaluation in experimental tumor systems. , 1962, Cancer chemotherapy reports.

[21]  M. Ashwood‐Smith.  Preservation of Mouse Bone Marrow at −79° C. with Dimethyl Sulphoxide , 1961, Nature.

[22]  E. Thomas,et al.  In vitro and in vivo assessment of the viability of dog marrow after storage. , 1960, Blood.