Asp-hemolysin Significance as a Virulence Factor with Biological Activities from Aspergillus fumigatus

Asp-hemolysin(AH)は,ペンギン肺から分離したAspergillus fumigatus (A. fumigatus) Fresenius-村松(FM)株が産生する易熱性タンパク質毒素で,各種動物赤血球に対しin vitroにおいて溶血作用を有する.成熟AHは,AH cDNA塩基配列および精製AHのN末端アミノ酸配列から126個のアミノ酸より成ることが推定される.またAHは,A.fumigatusの実験的感染に際し感染促進効果を示し,感染病巣中に産生される.さらにAHはin vivoにおいて,マウス肝細胞・心筋細胞の壊死性変化を惹起し,同時に腎臓および脳の動脈壁中膜に局在する.一方,in vitroにおいてAHはヒト多核白血球およびモルモット腹腔マクロファージに細胞毒性を示す.またA.fumigatus臨床分離株16株中,12株にAHタンパク質の発現を,また,13株にAH mRNAの発現を認めた.以上の結果,A.fumigatus感染における病変の形成,さらには進展に病原性因子の一つとしてAsp-hemolysinが関与する可能性が示唆された.

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