Nanomechanical biosensing with immunomagnetic separation

We report a biosensing method that combines immunomagnetic separation and nanomechanical detection. In this method, same magnetic beads that are used to “fish” biomolecules from complex mixtures enable deflection of a cantilever structure upon excitation by an oscillating magnetic field. Biotin-coated magnetic beads were used to capture and separate streptavidin from serum. Streptavidin loaded magnetic beads were exposed to a differential cantilever system whose sensing arm was functionalized with biotin. The magnetic force applied on the streptavidin-beads resulted in differential cantilever deflections that could be detected down to 0.26 Arms in air.

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