Central C-Fos Expression Following 20kHz/Ultrasound Induced Defence Behaviour in the Rat

Exposure of rats to aversive stimuli produces specific defence behaviour including the emission of 20-27kHz ultrasonic calls. Recent studies in this laboratory have shown that rats exposed to a 20kHz ultrasound tone display flight behaviour similar to that seen naturally, or following stimulation of brain regions associated with anxiety and defence. The present study examines the effect of ultrasound exposure on the central expression of the immediate early gene c-fos in the rat, in order to examine the brain structures activated by such behaviour. Ultrasound presentation produced rapid locomotor activity characteristic of defence behaviour, including brisk running and jumping behaviour. Animals showed dense c-fos like immunoreactivity in the dorsal periaqueductal grey matter, basolateral, medial, central amygdala, paraventricular thalamic nuclei and the dorsomedial nuclei of the hypothalamus, which was significantly greater than in either home-cage or arena control rats. These results suggest that exposure to artificially generated ultrasound can induce defence behaviour which is associated with activity in brain regions important in mediating aversion. This technique offers the potential of generating unconditioned aversive behaviour in rats in a non invasive way.

[1]  K. Mack,et al.  Induction of transcription factors in somatosensory cortex after tactile stimulation. , 1992, Brain research. Molecular brain research.

[2]  R. Handa,et al.  Induction of c-fos mRNA in the brain and anterior pituitary gland by a novel environment. , 1993, Neuroreport.

[3]  Donald W. Pfaff,et al.  Effects of daytime and nighttime stress on Fos-like immunoreactivity in the paraventricular nucleus of the hypothalamus, the habenula, and the posterior paraventricular nucleus of the thalamus , 1991, Brain Research.

[4]  D. Blanchard,et al.  Sex differences in the incidence and sonographic characteristics of antipredator ultrasonic cries in the laboratory rat (Rattus norvegicus). , 1992, Journal of comparative psychology.

[5]  G. Paxinos,et al.  The Rat Brain in Stereotaxic Coordinates , 1983 .

[6]  R. Naquet,et al.  Audiogenic seizures evoked in DBA/2 mice inducec-fos oncogene expression into subcortical auditory nuclei , 1990, Brain Research.

[7]  A. Snyder-Keller,et al.  Audiogenic seizures induce c-fos in a model of developmental epilepsy , 1992, Neuroscience Letters.

[8]  K. Johnson,et al.  Topographic patterns of brain activity in response to swim stress: assessment by 2-deoxyglucose uptake and expression of Fos-like immunoreactivity , 1993, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[9]  C H Beck,et al.  Conditioned fear-induced changes in behavior and in the expression of the immediate early gene c-fos: with and without diazepam pretreatment , 1995, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[10]  E. Rouiller,et al.  Mapping of c-fos expression elicited by pure tones stimulation in the auditory pathways of the rat, with emphasis on the cochlear nucleus , 1992, Neuroscience Letters.

[11]  Mark A. Smith,et al.  Induction of c-fos mRNA in rat brain by conditioned and unconditioned stressors , 1992, Brain Research.

[12]  G. Sales,et al.  Ultrasound and aggressive behaviour in rats and other small mammals. , 1972, Animal behaviour.

[13]  R. Depoortère,et al.  What brain structures are active during emotions? Effects of brain stimulation elicited aversion on c-fos immunoreactivity and behavior , 1993, Behavioural Brain Research.

[14]  E. Nestler,et al.  Induction of the c-fos proto-oncogene during opiate withdrawal in the locus coeruleus and other regions of rat brain , 1990, Brain Research.

[15]  B. Rabin,et al.  Induction of c-Fos immunoreactivity in the rat forebrain by conditioned and unconditioned aversive stimuli , 1992, Brain Research.

[16]  T. Hökfelt,et al.  Expression of c-Fos immunoreactivity in transmitter-characterized neurons after stress. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[17]  S. Brudzyński,et al.  Behavioural responses of laboratory rats to playback of 22 kHz ultrasonic calls , 1995, Physiology & Behavior.

[18]  E. Bullitt.  Expression of C‐fos‐like protein as a marker for neuronal activity following noxious stimulation in the rat , 1990, The Journal of comparative neurology.

[19]  D. C. Blanchard,et al.  Twenty-two kHz alarm cries to presentation of a predator, by laboratory rats living in visible burrow systems , 1991, Physiology & Behavior.

[20]  Michael Davis,et al.  Induction of the c-fos proto-oncogene in rat amygdala during unconditioned and conditioned fear , 1991, Brain Research.

[21]  R. Faull,et al.  The use of c-fos as a metabolic marker in neuronal pathway tracing , 1989, Journal of Neuroscience Methods.

[22]  T. Herdegen,et al.  c-FOS-like immunoreactivity in rat brainstem neurons following noxious chemical stimulation of the nasal mucosa , 1991, Neuroscience.

[23]  D. Blanchard,et al.  The characterization and modelling of antipredator defensive behavior , 1990, Neuroscience & Biobehavioral Reviews.

[24]  K. Noguchi,et al.  Stress-induced c-fos expression in the rat brain: activation mechanism of sympathetic pathway , 1993, Brain Research Bulletin.

[25]  George R. Breese,et al.  Neuroanatomical characterization of Fos induction in rat behavioral models of anxiety , 1996, Brain Research.

[26]  R. Leslie,et al.  Serotonin2/1 C receptor activation causes a localized expression of the immediate-early gene c-fos in rat brain: evidence for involvement of dorsal raphe nucleus projection fibres , 1993, Neuroscience.

[27]  Computer analysis and quantification of periaqueductal grey-induced defence behaviour , 1995, Journal of Neuroscience Methods.

[28]  W. Redfern,et al.  A search for brain stem cell groups integrating the defence reaction in the rat. , 1986, The Journal of physiology.