Antibacterial and cytotoxic activities of the chloroform extract of the stem bark of Crataeva nurvala (Bengali nameBorun; FamilyCapparidaceae) were studied. Antibacterial activity was determined against two Gram positive (Bacillus cereus, Bacillus megaterium,) and four Gram negative (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella boydii) human pathogenic bacteria by disc diffusion method. The range of zone of inhibition of the extract was 8.3 to 22.1 mm. The extract showed no activity against Bacillus megaterium. Brine shrimp lethality bioassay method was used to determine the toxicity of the plant extract and ampicillin trihydrate was used as positive control. The LC50 values of standard ampicillin trihydrate and extract were 11.48 μg/ml and 14.12 μg/ml respectively. In addition serial dilution technique was used to determine the minimum inhibitory concentration (MIC) of the plant extract. The MIC values were recorded 62.5 μg/ml against both Escherichia coli and Bacillus cereus. INTRODUCTION: The frequency of life threatening infections caused by pathogenic micro-organisms has increased worldwide and is becoming an important cause of morbidity and mortality in immunocompromised patients in developing countries . Although huge number of antimicrobial agents has been discovered, the pathogenic micro-organisms are developing resistance against these agents day by day. In third world countries irrational use of antimicrobial agents is the major cause of such resistance. In recent year, attempts have been made to investigate the indigenous drugs against infectious diseases . Research in the field of indigenous plants is a significant aspect of developing safer antimicrobial principles through isolation, characterization, identification and biological studies. The plant Crataeva nurvala belonging to the family Capparidaceae is a leafy small to medium sized soft wooded tree with fragrant white flowers and oblong to rounded hard fruits, grows on the banks of canals, rivers, lakes etc. It is an evergreen tree growing widely in all parts of Bangladesh, Pakistan, India, Philippine, South America, China, Africa etc . The bark of the tree is an important drug for problem affecting the kidneys and bladder. It is especially effective in the urinary complaints, kidney and bladder stones (inhibit the formation of stones), fever, vomiting and gastric irritation. It also acts as contraceptive and oxytocic; juice of the bark is given to women after childbirth. Barks are also laxative and lithontriptic. They increase appetite and billiary secretion .
[1]
T. T. Shelkea,et al.
NEPHROPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF STEM BARKS OF CRATAEVA NURVULA BUCH HUM
,
2011
.
[2]
Rahman,et al.
CHARACTERIZATION AND ANTIMICROBIAL ACTIVITIES OF A PHENOLIC ACID DERIVATIVE PRODUCED BY STREPTOMYCES BANGLADESHIENSIS A NOVEL SPECIS COLLECTED IN BANGLADESH
,
2006
.
[3]
A. Shirwaikar,et al.
Effect of lupeol isolated from Crataeva nurvala Buch.-Ham. stem bark extract against free radical induced nephrotoxicity in rats.
,
2004,
Indian journal of experimental biology.
[4]
Sayeed,et al.
TERPENES FROM BARK OF ZANTHOXYLUM BUDRUNGA AND THEIR CYTOTOXIC ACTIVITIES
,
2002
.
[5]
M. Haque,et al.
IN VITRO ANTIBACTERIAL ACTIVITY OF THE COMPOUNDS OF TRAPA BISPINOSA ROXB
,
2001
.
[6]
A. Ghani.
Medicinal plants of Bangladesh: Chemical constituents and uses
,
1998
.
[7]
A. Giulietti,et al.
Bioactivity of certain medicinal latexes used by the Ese'ejas.
,
1995,
Journal of ethnopharmacology.
[8]
E. Gürkan,et al.
CYTOTOXICITY ASSAY OF SOME PAPAVER ALKALOIDS USING ARTEMIA SALINA (BRINE SHRIMP)
,
1995
.
[9]
M. Caviedes,et al.
Cytotoxic activity of Retama sphaerocarpa
,
1995
.
[10]
Arthur I. Vogel,et al.
Vogel's Textbook of Quantitative Chemical Analysis
,
1989
.
[11]
D. E. Nichols,et al.
Brine shrimp: a convenient general bioassay for active plant constituents.
,
1982,
Planta medica.
[12]
G. Persoone.
The Brine shrimp Artemia : proceedings of the international symposium on the brine shrimp Artemia Salina. Corpus Christi, Texas, USA, August 20-23, 1979.
,
1980
.
[13]
E. Henninot.
[Antibiotics. Introduction].
,
1972,
Journal des sciences medicales de Lille.
[14]
A. Bauer,et al.
Antibiotic susceptibility testing by a standardized single disk method.
,
1966,
Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists.
[15]
J. Hooker.
Flora of British India, Vol. IV.
,
1879
.