Induced resistance of Potato (Solanum tuberosum L.) toward Ralstonia solanacearum disease with combination of several bio-control microbes

The research aims to study the influence of bio-control microbes of Trichoderma viride., Streptomyces sp. and Pseudomonas fluorescens on the resistence of potato crop toward Ralstonia solanacearum disease and to get a combination of the best bio-control microbes to increase potato growth and yield. The research was conducted on February to June 2013 at the screen house of School of Agriculture of Unisma. As the research treatment, the selected isolates of Trichoderma viride, Streptomyces sp ., and Pseudomonas fluorescens were applied as a single treatment or in combination. The tested treatments were arranged in completely randomized block design (RAK) with nine treatments and three repetitions. Research result showed that treatment of application with Pseudomonas fluorescens, either as single treatment or in combination with Streptomyces sp. and Trichoderma viride + Streptomyces sp. , has potential to induce resistance toward Ralstonia solanacearum disease on potato. It is showed by its ability to extend the incubation period of 11 - 13 days, reduce the disease incidence of 72.01 - 76.55%, reduce population density of R. solanacearum of 50.79% - 57.81%, increase the total phenol of crop's leaves, increase crop's length 7.09 - 12.49% and increase tuber fresh weight of 149.29 - 150.27%.

[1]  A. L. Abadi,et al.  ENHANCEMENT IN EFFECTIVENESS OF ANTAGONISTIC MICROBE BY MEANS OF MICROBIAL COMBINATION TO CONTROL Ralstonia solanacearum ON POTATO PLANTED IN MIDDLE LATITUDE , 2013 .

[2]  J. Vanderleyden,et al.  Auxin and plant-microbe interactions. , 2011, Cold Spring Harbor perspectives in biology.

[3]  C. Pieterse,et al.  Networking by small-molecule hormones in plant immunity. , 2009, Nature chemical biology.

[4]  Daciana Ciocan,et al.  PLANT PRODUCTS AS ANTIMICROBIAL AGENTS , 2007 .

[5]  S. Mathiyazhagan,et al.  Induction of phenolics and defense-related enzymes in coconut (Cocos nucifera L.) roots treated with biocontrol agents , 2006 .

[6]  E. Tsavkelova,et al.  Microbial producers of plant growth stimulators and their practical use: A review , 2006, Applied Biochemistry and Microbiology.

[7]  R. M. Goodman,et al.  Systemic acquired resistance and induced systemic resistance in conventional agriculture , 2004 .

[8]  A. Rincón,et al.  Biocontrol mechanisms of Trichoderma strains. , 2004, International microbiology : the official journal of the Spanish Society for Microbiology.

[9]  I. Dubery,et al.  Soluble and wall-bound phenolics and phenolic polymers in Musa acuminata roots exposed to elicitors from Fusarium oxysporum f.sp. cubense. , 2003, Phytochemistry.

[10]  C. H. Beckman Phenolic-storing cells : keys to programmed cell death and periderm formation in wilt disease resistance and in general defence responses in plants? , 2000 .

[11]  M. M. Cowan Plant Products as Antimicrobial Agents , 1999, Clinical Microbiology Reviews.

[12]  R. Kneusel,et al.  Plant cell wall reinforcement in the disease resistance response : molecular composition and regulation , 1995 .

[13]  Laurence V. Madden,et al.  Introduction to Plant Disease Epidemiology , 1990 .

[14]  R. Bruce,et al.  Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean. , 1989, Plant physiology.

[15]  R. E. Campbell,et al.  Biological control of microbial plant pathogens , 1989, Plant Growth Regulation.

[16]  David M. Weller,et al.  Biological control of soilborne plant pathogens in the rhizosphere with bacteria , 1988 .

[17]  A. Melser POST-GRADUATE PROGRAM. , 1965, Bulletin / Monmouth-Ocean County Dental Society.

[18]  R. Boro,et al.  Characterization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam , 2004 .

[19]  J. Kloepper,et al.  Role of biological preparations in enhancement of rice seedling growth and grain yield , 2002 .

[20]  Peter Jeffries,et al.  Biotic Interactions in Plant-Pathogen Associations , 2002 .

[21]  L. Samuelson,et al.  Influence of two plant growth-promoting rhizobacteria on loblolly pine root respiration and IAA activity , 2001 .

[22]  Z. Kiraly,et al.  The Biochemistry and Physiology of Plant Disease , 1986 .

[23]  J. Callow,et al.  Transcription and translation in the diseased plant , 1983 .