5G networks will constitute a complete transformation in the ICT domain by enabling the deployment of vertical services within the network infrastructures, based on extensive use of network softwarization and programmability. This shift will trigger and facilitate the transformation of existing stakeholders’ roles, as well as the interactions between multiple stakeholders from the traditionally separated markets. The 5G-PPP project MATILDA aims at delivering a holistic 5G end-to-end services operational framework, including 5G-ready applications lifecycle management from development to deployment over 5G network infrastructures. This paper aims at providing a refined and extended vision of the 5G business roles and their interactions and based on these at defining business applicability of the MATILDA project, with special focus on the project’s value proposition addressing the Telecommunication Service Providers.