Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA

The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft agar. The phenotypic alteration in the transformed cells was reversed by the addition of anti-human bFGF antibodies to the medium. These results suggest that the cellular transformation mediated by bFGF is caused by autocrine stimulation with secreted bFGF molecules.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  D. Gospodarowicz,et al.  Fibroblast Growth Factor: Involvement in Early Embryonic Development and Ovarian Function , 1989, Critical reviews in oncogenesis.

[3]  M. Seno,et al.  Expression of cDNA encoding human basic fibroblast growth factor in E. coli. , 1987, Biochemical and biophysical research communications.

[4]  H. Onda,et al.  The establishment of IL-2 producing cells by genetic engineering. , 1987, Cell structure and function.

[5]  T. Kurokawa,et al.  Cloning and expression of cDNA encoding human basic fibroblast growth factor , 1987, FEBS letters.

[6]  R. Tjian,et al.  Activation of transcription by two factors that bind promoter and enhancer sequences of the human metallothionein gene and SV40 , 1987, Nature.

[7]  R. Weinberg,et al.  Construction of a novel oncogene based on synthetic sequences encoding epidermal growth factor. , 1987, Science.

[8]  J. Fiddes,et al.  Human basic fibroblast growth factor: nucleotide sequence and genomic organization. , 1986, The EMBO journal.

[9]  J. Fiddes,et al.  Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor. , 1986, Science.

[10]  D. Goeddel,et al.  Expression in rat fibroblasts of a human transforming growth factor-α cDNA results in transformation , 1986, Cell.

[11]  D. Gospodarowicz,et al.  Basic and acidic fibroblast growth factors interact with the same cell surface receptors. , 1986, The Journal of biological chemistry.

[12]  R. Lang,et al.  Expression of a hemopoietic growth factor cDNA in a factor-dependent cell line results in autonomous growth and tumorigenicity , 1985, Cell.

[13]  W. Schaffner,et al.  Metal‐dependent SV40 viruses containing inducible enhancers from the upstream region of metallothionein genes. , 1985, The EMBO journal.

[14]  C. Heldin,et al.  Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus , 1985, Nature.

[15]  S. Aaronson,et al.  The v‐sis/PDGF‐2 transforming gene product localizes to cell membranes but is not a secretory protein. , 1985, The EMBO journal.

[16]  C. Heldin,et al.  Coexpression of a PDGF-like growth factor and PDGF receptors in a human osteosarcoma cell line: Implications for autocrine receptor activation , 1984, Cell.

[17]  S. Aaronson,et al.  Expression of the normal human sis/PDGF-2 coding sequence induces cellular transformation , 1984, Cell.

[18]  T. Deuel,et al.  Transforming protein of simian sarcoma virus stimulates autocrine growth of SSV-transformed cells through PDGF cell-surface receptors , 1984, Cell.

[19]  C. Heldin,et al.  Growth factors: Mechanism of action and relation to oncogenes , 1984, Cell.

[20]  Paul Stroobant,et al.  Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus , 1983, Nature.

[21]  R. Palmiter,et al.  Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs , 1981, Cell.

[22]  G. Gill,et al.  Increased phosphotyrosine content and inhibition of proliferation in EGF-treated A431 cells , 1981, Nature.

[23]  M. Sporn,et al.  Autocrine secretion and malignant transformation of cells. , 1980, The New England journal of medicine.

[24]  B. Ozanne,et al.  Kirsten murine sarcoma virus transformed cell lines and a spontaneously transformed rat cell‐line produce transforming factors , 1980, Journal of cellular physiology.

[25]  T. Kakunaga,et al.  Cell variants showing differential susceptibility to ultraviolet light--induced transformation. , 1980, Science.

[26]  Bernard D. Davis,et al.  The mechanism of protein secretion across membranes , 1980, Nature.

[27]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[28]  Tom Maniatis,et al.  Transformation of mammalian cells with genes from procaryotes and eucaryotes , 1979, Cell.

[29]  G. Todaro,et al.  Growth factors from murine sarcoma virus-transformed cells. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[30]  H. Boedtker,et al.  RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. , 1977, Biochemistry.

[31]  D. Gospodarowicz,et al.  Effect of a fibroblast growth factor, insulin, dexamethasone, and serum on the morphology of BALB/c 3T3 cells. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[32]  D. Gospodarowicz,et al.  Stimulation of division of sparse and confluent 3T3 cell populations by a fibroblast growth factor, dexamethasone, and insulin. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[33]  J. Folkman,et al.  Angiogenic factors. , 1987, Science.

[34]  N. Ling,et al.  Molecular characterization of fibroblast growth factor: distribution and biological activities in various tissues. , 1986, Recent progress in hormone research.

[35]  Anita B. Roberts,et al.  Autocrine growth factors and cancer , 1985, Nature.

[36]  John D. Minna,et al.  Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer , 1985, Nature.

[37]  A. Rizzino,et al.  The growth of cells in serum-free hormone-supplemented media. , 1979, Methods in enzymology.

[38]  D. Gospodarowicz,et al.  Growth factors in mammalian cell culture. , 1976, Annual review of biochemistry.