Advances in gentle immunoaffinity chromatography.

Immunoaffinity chromatography is one of the most powerful fractionation steps available for protein purification; however, it is often difficult to elute bound protein without using harsh or denaturing elution conditions. The development of methods to identify monoclonal antibodies that bind antigens tightly, but release under gentle, non-denaturing conditions has made possible the immunoaffinity purification of labile, multisubunit enzyme complexes with high yield and high specific activity. This work has implications for emerging proteomic applications, allowing identification of new protein-protein interaction partners, retention of biological activity and the isolation of protein complexes more amenable to crystallization and structure determination.

[1]  K. Thorn,et al.  A novel method of affinity‐purifying proteins using a bis‐arsenical fluorescein , 2008, Protein science : a publication of the Protein Society.

[2]  D. Strickland,et al.  Process Implications for Metal‐Dependent Immunoaffinity Interactions , 1989 .

[3]  E. Hochuli,et al.  New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. , 1987, Journal of chromatography.

[4]  Marietta Y. W. T. Lee,et al.  Immunoaffinity purification of DNA polymerase δ1 , 1995 .

[5]  R. Kornberg,et al.  A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3 , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[6]  M. Böcher,et al.  A new peptide-affinity tag for the detection and affinity purification of recombinant proteins with a monoclonal antibody. , 2000, Journal of immunological methods.

[7]  N. Thompson,et al.  Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. , 1989, The Journal of biological chemistry.

[8]  M. Uhlén,et al.  Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. , 1997, Protein expression and purification.

[9]  E. Harlow,et al.  Antibodies: A Laboratory Manual , 1988 .

[10]  N. Thompson,et al.  Immunoaffinity purification of RNA polymerase II and transcription factors using polyol-responsive monoclonal antibodies. , 1996, Methods in enzymology.

[11]  B. Séraphin,et al.  A generic protein purification method for protein complex characterization and proteome exploration , 1999, Nature Biotechnology.

[12]  N. Thompson,et al.  Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase. , 1992, Biochemistry.

[13]  N. Thompson,et al.  Purification and lipid-layer crystallization of yeast RNA polymerase II. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[14]  B. Slatko,et al.  An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. , 1988, Gene.

[15]  N. Thompson,et al.  A novel collection of accessory factors associated with yeast RNA polymerase II. , 1996, Protein expression and purification.

[16]  R. Burgess,et al.  Strategies for Protein Purification and Characterization A Laboratory Course Manual , 1996 .

[17]  M. Weller,et al.  Immunochromatographic techniques – a critical review , 2000, Fresenius' journal of analytical chemistry.

[18]  D. Gibson,et al.  Rapid purification of the oxygenase component of toluene dioxygenase from a polyol-responsive monoclonal antibody , 1996, Applied and environmental microbiology.

[19]  M. Yates,et al.  (Immuno)affinity chromatography: a versatile tool for fast and selective purification, concentration, isolation and analysis. , 2001, Journal of pharmaceutical and biomedical analysis.

[20]  R. Bartholomew,et al.  Immunoaffinity Chromatography with Monoclonal Antibodies , 1983 .

[21]  Grant J. Jensen,et al.  Yeast RNA Polymerase II at 5 Å Resolution , 1999, Cell.

[22]  N. Thompson,et al.  Immunoaffinity purification of the RAP30 subunit of human transcription factor IIF. , 1999, Protein expression and purification.

[23]  N. Thompson,et al.  Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody. , 1990, The Journal of biological chemistry.

[24]  S A Lesley,et al.  High-throughput proteomics: protein expression and purification in the postgenomic world. , 2001, Protein expression and purification.

[25]  N. Thompson,et al.  The ABL-MYC retrovirus generates antigen-specific plasmacytomas by in vitro infection of activated B lymphocytes from spleen and other murine lymphoid organs. , 1996, Journal of immunological methods.