论文信息 - Report on the Verification of the Performance of MON 89034, 1507 and NK603 Event-specific Methods on the Maize Event MON 89034 x 1507 x NK603 Using Real-Time PCR. Validation Report and Protocols

Report on the Verification of the Performance of MON 89034, 1507 and NK603 Event-specific Methods on the Maize Event MON 89034 x 1507 x NK603 Using Real-Time PCR. Validation Report and Protocols

The European Union Reference Laboratory for GM Food and Feed EURL-GMFF), established by Regulation (EC) No 1829/2003, has carried out a verification study to assess the performance of three quantitative eventspecific methods on the maize event MON 89034 x 1507 x NK603 (unique identifier MON-89Ø34-3 x DASØ15Ø7-1 x MON-ØØ6Ø3-6) which combines the MON 89034, 1507 and NK603 transformation events. The three methods have been validated individually on single-trait events, to detect and quantify each event in maize samples. This study was conducted according to internationally accepted guidelines (1, 2). In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and to Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Monsanto and Dow AgroSciences provided the detection methods and the control samples: genomic DNA from homogenised seeds of MON 89034 x 1507 x NK603 maize (60-B) and from homogenised seeds of conventional maize (10001262-V). The EURL-GMFF prepared the verification samples (calibration samples and blind samples at different GM percentages [DNA/DNA]). The results of the verification study were evaluated with reference to ENGL method performance requirements (http://gmo-crl.jrc.ec.europa.eu/guidancedocs.htm) and to the validation results on the individual parental events (http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm). The results of this EURL-GMFF verification study are publicly available at http://gmo-crl.jrc.ec.europa.eu/. How to obtain EU publications Our priced publications are available from EU Bookshop (http://bookshop.europa.eu), where you can place an order with the sales agent of your choice. The Publications Office has a worldwide network of sales agents. You can obtain their contact details by sending a fax to (352) 29 29-42758. The mission of the JRC is to provide customer-driven scientific and technical support for the conception, development, implementation and monitoring of EU policies. As a service of the European Commission, the JRC functions as a reference centre of science and technology for the Union. Close to the policy-making process, it serves the common interest of the Member States, while being independent of special interests, whether private or national. LB -N A -2401-EN -C

Mazzara Marco | Van Den Eede Guy | Grazioli Emanuele | Luque Perez Encarnacion

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