SNAP receptors implicated in vesicle targeting and fusion

The N-ethylmaleimide-sensitive fusion protein (NSF) and the soluble NSF attachment proteins (SNAPs) appear to be essential components of the intracellular membrane fusion apparatus. An affinity purification procedure based on the natural binding of these proteins to their targets was used to isolate SNAP receptors (SNAREs) from bovine brain. Remarkably, the four principal proteins isolated were all proteins associated with the synapse, with one type located in the synaptic vesicle and another in the plasma membrane, suggesting a simple mechanism for vesicle docking. The existence of numerous SNARE-related proteins, each apparently specific for a single kind of vesicle or target membrane, indicates that NSF and SNAPs may be universal components of a vesicle fusion apparatus common to both constitutive and regulated fusion (including neurotransmitter release), in which the SNAREs may help to ensure vesicle-to-target specificity.

[1]  J. Skene,et al.  The 25 kDa synaptosomal-associated protein SNAP-25 is the major methionine-rich polypeptide in rapid axonal transport and a major substrate for palmitoylation in adult CNS , 1992, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[2]  J. Rothman,et al.  Dissection of a single round of vesicular transport: Sequential intermediates for intercisternal movement in the Golgi stack , 1989, Cell.

[3]  T. Südhof,et al.  Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C , 1990, Nature.

[4]  T. Reese,et al.  The organization of cytoplasm at the presynaptic active zone of a central nervous system synapse , 1988, Neuron.

[5]  J. Rothman,et al.  Transport of vesicular stomatitis virus glycoprotein in a cell-free extract. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[6]  R. Scheller,et al.  Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system. , 1989, The Journal of biological chemistry.

[7]  M Linial,et al.  Cellular and molecular biology of the presynaptic nerve terminal. , 1991, Annual review of neuroscience.

[8]  D. Gallwitz,et al.  Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily , 1991, Molecular and cellular biology.

[9]  S. Munro,et al.  A C-terminal signal prevents secretion of luminal ER proteins , 1987, Cell.

[10]  E. Harlow,et al.  Antibodies: A Laboratory Manual , 1988 .

[11]  R Llinás,et al.  Relationship between presynaptic calcium current and postsynaptic potential in squid giant synapse. , 1981, Biophysical journal.

[12]  H. Pelham,et al.  SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex , 1992, The Journal of cell biology.

[13]  J. Rothman,et al.  SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast , 1990, Cell.

[14]  R. Schekman,et al.  The yeast SEC17 gene product is functionally equivalent to mammalian alpha-SNAP protein. , 1992, The Journal of biological chemistry.

[15]  S. Emr,et al.  Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. , 1991 .

[16]  J. Rothman,et al.  Purification of three related peripheral membrane proteins needed for vesicular transport. , 1990, The Journal of biological chemistry.

[17]  J. Rothman,et al.  Expression and purification of recombinant N-ethylmaleimide-sensitive fusion protein from Escherichia coli. , 1992, Methods in enzymology.

[18]  J. Rothman,et al.  A multisubunit particle implicated in membrane fusion , 1992, The Journal of cell biology.

[19]  J. Rothman,et al.  Possible role for fatty acyl-coenzyme A in intracellular protein transport , 1987, Nature.

[20]  E. Chen,et al.  A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast , 1989, Nature.

[21]  J. Rothman,et al.  SNAP family of NSF attachment proteins includes a brain-specific isoform , 1993, Nature.

[22]  P. Tempst,et al.  Internal sequence analysis of proteins separated on polyacrylamide gels at the submicrogram level: Improved methods, applications and gene cloning strategies , 1990, Electrophoresis.

[23]  P. Mancini,et al.  SEC22 and SLY2 are identical , 1992, Molecular and cellular biology.

[24]  T. Südhof,et al.  A synaptic vesicle membrane protein is conserved from mammals to Drosophila , 1989, Neuron.

[25]  S. Ferro-Novick,et al.  Bos1p, a membrane protein required for ER to Golgi transport in yeast, co‐purifies with the carrier vesicles and with Bet1p and the ER membrane. , 1992, The EMBO journal.

[26]  T. Südhof,et al.  Synaptotagmin: a calcium sensor on the synaptic vesicle surface. , 1992, Science.

[27]  S. Ferro-Novick,et al.  The BOS1 gene encodes an essential 27-kD putative membrane protein that is required for vesicular transport from the ER to the Golgi complex in yeast , 1991, The Journal of cell biology.

[28]  T. Südhof,et al.  Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2. , 1990, The Journal of biological chemistry.

[29]  J. Rothman,et al.  Fatty acylation promotes fusion of transport vesicles with Golgi cisternae , 1990, The Journal of cell biology.

[30]  J. Rothman,et al.  A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack , 1992, The Journal of cell biology.

[31]  R. Scheller,et al.  Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones. , 1992, Science.

[32]  J. Rothman,et al.  Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[33]  J. Rothman,et al.  Binding of an N-ethylmaleimide-sensitive fusion protein to Golgi membranes requires both a soluble protein(s) and an integral membrane receptor , 1989, The Journal of cell biology.

[34]  J. Rothman,et al.  Uncoating ATPase is a member of the 70 kilodalton family of stress proteins , 1986, Cell.

[35]  F E Bloom,et al.  The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulations , 1989, The Journal of cell biology.

[36]  J. Rothman,et al.  Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes. , 1992, The Journal of biological chemistry.

[37]  K. Becherer,et al.  Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae , 1991, Molecular and cellular biology.

[38]  R. Kelly.  Pathways of protein secretion in eukaryotes. , 1985, Science.

[39]  R. Schekman,et al.  Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway , 1990, Cell.

[40]  K. Miller,et al.  Differential expression of the p65 gene family , 1991, Neuron.

[41]  R. Schekman,et al.  Order of events in the yeast secretory pathway , 1981, Cell.

[42]  P. Tempst,et al.  Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis. , 1989, Analytical biochemistry.

[43]  J. Rothman,et al.  Reconstitution of the transport of protein between successive compartments of the golgi measured by the coupled incorporation of N-acetylglucosamine , 1984, Cell.

[44]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[45]  G K Lewis,et al.  Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product , 1985, Molecular and cellular biology.

[46]  T. Südhof,et al.  A small GTP-binding protein dissociates from synaptic vesicles during exocytosis , 1991, Nature.

[47]  F Benfenati,et al.  Synaptic vesicle phosphoproteins and regulation of synaptic function. , 1993, Science.

[48]  B. Chait,et al.  Cinnamic acid derivatives as matrices for ultraviolet laser desorption mass spectrometry of proteins. , 1989, Rapid communications in mass spectrometry : RCM.

[49]  Benjamin S. Glick,et al.  Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack , 1988, Cell.

[50]  Thomas C. Südhof,et al.  Proteins of synaptic vesicles involved in exocytosis and membrane recycling , 1991, Neuron.

[51]  F. Benfenati,et al.  Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin , 1992, Nature.

[52]  G. Schlenstedt,et al.  A large presecretory protein translocates both cotranslationally, using signal recognition particle and ribosome, and post-translationally, without these ribonucleoparticles, when synthesized in the presence of mammalian microsomes. , 1990, The Journal of biological chemistry.

[53]  G. Palade,et al.  Intracellular aspects of the process of protein synthesis. , 1975, Science.