Biochemical characterization of blood orange, sweet orange, lemon, bergamot and bitter orange.

This paper reports on the composition of aroma compounds and fatty acids and some physico-chemical parameters (juice percentage, acidity and total sugars) in five varieties of citrus: blood orange, sweet orange, lemon, bergamot and bitter orange. Volatile compounds and methyl esters have been analyzed by gas chromatography. Limonene is the most abundant compound of monoterpene hydrocarbons for all of the examined juices. Eighteen fatty acids have been identified in the studied citrus juices, their quantification points out that unsaturated acids predominate over the saturated ones. Mean concentration of fatty acids varies from 311.8 mg/l in blood orange juice to 678 mg/l in bitter orange juice.

[1]  G. Tucker,et al.  Biochemistry of Fruit Ripening , 1993, Springer Netherlands.

[2]  B. Fallico,et al.  Fatty acids of Italian blood orange juices , 1998 .

[3]  M. Petersen,et al.  Discrimination between freshly made and stored reconstituted orange juice using GC odour profiling and aroma values , 1998 .

[4]  B. Fallico,et al.  Flavor components of Italian Orange Juices , 1998 .

[5]  L. Mondello,et al.  Automated HPLC-HRGC , 1994 .

[6]  R. Tressl,et al.  Biogenesis of Aroma Compounds through Acyl Pathways , 1986 .

[7]  J. Estienne,et al.  Differentiation of citrus juices by factorial discriminant analysis using liquid chromatography of flavanone glycosides , 1994 .

[8]  R. D. Carter,et al.  Ultrafiltration of orange juice: effect on soluble solids, suspended solids, and aroma , 1992 .

[9]  R. D. Carter,et al.  Ultrafiltration of orange juice , 1992 .

[10]  C. S. Chen,et al.  Flavor losses in orange juice during ultrafiltration and subsequent evaporation , 1996 .

[11]  L. Mondello,et al.  Automated HPLC‐HRGC: A powerful method for essential oils analysis. Part V. identification of terpene hydrocarbons of bergamot, lemon, mandarin, sweet orange, bitter orange, grapefruit, clementine and mexican lime oils by coupled HPLC‐HRGC‐MS(ITD) , 1995 .

[12]  R. L. Coleman,et al.  Composition of Orange Essence Oil , 1969 .

[13]  J. Navarro,et al.  Characterization of Spanish orange juice for variables used in purity control , 1989 .

[14]  B. Vick,et al.  Lipoxygenase and hydroperoxide lyase in germinating watermelon seedlings. , 1976, Plant physiology.

[15]  B. Vick,et al.  The Lipoxygenase Pathway , 1987 .

[16]  S. Campisi,et al.  Lipids in citrus fruit juice. 1. Lipid content during orange ripening in eastern Sicily , 1987 .

[17]  G. Dugo,et al.  On the genuineness of citrus essential oils. Part XLI. Italian bitter orange essential oil: Composition and detection of contamination and additions of oils and terpenes of sweet orange and of lemon , 1993 .

[18]  E. Mercer,et al.  Recent Advances in the Chemistry and Biochemistry of Plant Lipids , 1977 .

[19]  C. Rice-Evans,et al.  Antioxidant properties of phenolic compounds , 1997 .

[20]  Robert Willard Hodgson,et al.  Horticultural varieties of citrus , 1967 .

[21]  J. Carreau,et al.  Adaptation of a macro-scale method to the micro-scale for fatty acid methyl transesterification of biological lipid extracts , 1978 .

[22]  M. Meireles,et al.  Recovery of aroma compounds from orange essential oil , 2000 .