Three dimensional bioprinting technology: Applications in pharmaceutical and biomedical area.

3D bioprinting is a technology based on the principle of three-dimensional printing of designed biological materials, which has been widely used recently. The production of biological materials, such as tissues, organs, cells and blood vessels with this technology is alternative and promising approach for organ and tissue transplantation. Apart from tissue and organ printing, it has a wide range of usage, such as in vitro/in vivo modeling, production of drug delivery systems and, drug screening. However, there are various restrictions on the use of this technology. In this review, the process steps, classification, advantages, limitations, usage and application areas of 3D bioprinting technology, materials and auxiliary materials used in this technology are discussed.

[1]  Igor M. Sauer,et al.  Liver support strategies: cutting-edge technologies , 2014, Nature Reviews Gastroenterology &Hepatology.

[2]  F. Lin,et al.  Three-dimensional Gelatin and Gelatin/Hyaluronan Hydrogel Structures for Traumatic Brain Injury , 2007 .

[3]  R. Ramakrishnan,et al.  3D printed polycarbonate reinforced acrylonitrile–butadiene–styrene composites: Composition effects on mechanical properties, micro-structure and void formation study , 2019, Journal of Mechanical Science and Technology.

[4]  N. Abolfathi,et al.  Evaluation of fibrin-gelatin hydrogel as biopaper for application in skin bioprinting: An in-vitro study. , 2017, Bio-medical materials and engineering.

[5]  Wei Sun,et al.  Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation , 2016, Scientific Reports.

[6]  Renata Jachowicz,et al.  3D Printing in Pharmaceutical and Medical Applications – Recent Achievements and Challenges , 2018, Pharmaceutical Research.

[7]  S. Takayama,et al.  Rapid generation of multiplexed cell cocultures using acoustic droplet ejection followed by aqueous two-phase exclusion patterning. , 2012, Tissue engineering. Part C, Methods.

[8]  Hong Zhou,et al.  Biomaterial selection for tooth regeneration. , 2011, Tissue engineering. Part B, Reviews.

[9]  Tongkui Cui,et al.  Rapid Prototyping a Double-layer Polyurethane—collagen Conduit and its Schwann Cell Compatibility , 2009 .

[10]  FischerHorst,et al.  The stiffness and structure of three-dimensional printed hydrogels direct the differentiation of mesenchymal stromal cells toward adipogenic and osteogenic lineages. , 2015 .

[11]  Hui Lin,et al.  Development of 3D bioprinting: From printing methods to biomedical applications , 2019, Asian journal of pharmaceutical sciences.

[12]  Vladimir Mironov,et al.  Towards organ printing: engineering an intra-organ branched vascular tree , 2010, Expert opinion on biological therapy.

[13]  Akhilesh K. Gaharwar,et al.  Polymers for Bioprinting , 2015 .

[14]  M. Lythgoe,et al.  Chemically Treated 3D Printed Polymer Scaffolds for Biomineral Formation , 2018, ACS omega.

[15]  Dong-Woo Cho,et al.  3D Printed Tissue Models: Present and Future. , 2016, ACS biomaterials science & engineering.

[16]  P. Bártolo,et al.  Additive manufacturing of tissues and organs , 2012 .

[17]  Ian Gibson,et al.  Additive manufacturing technologies : 3D printing, rapid prototyping, and direct digital manufacturing , 2015 .

[18]  B. Derby,et al.  Delivery of human fibroblast cells by piezoelectric drop-on-demand inkjet printing. , 2008, Biomaterials.

[19]  Kristin Syverud,et al.  3D Bioprinting of Carboxymethylated-Periodate Oxidized Nanocellulose Constructs for Wound Dressing Applications , 2015, BioMed research international.

[20]  Bing Chen,et al.  3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers , 2015, Biofabrication.

[21]  Xiaohong Wang,et al.  Incorporation of DMSO and dextran-40 into a gelatin/alginate hydrogel for controlled assembled cell cryopreservation. , 2010, Cryobiology.

[22]  L. Gu,et al.  Mechanical Characterizations of 3D-printed PLLA/Steel Particle Composites , 2018, Materials.

[23]  Jerome Ritz,et al.  The elusive nature and function of mesenchymal stem cells , 2011, Nature Reviews Molecular Cell Biology.

[24]  Rui L. Reis,et al.  Alternative tissue engineering scaffolds based on starch: processing methodologies, morphology, degradation and mechanical properties , 2002 .

[25]  Daniel Therriault,et al.  Properties of polylactide inks for solvent-cast printing of three-dimensional freeform microstructures. , 2014, Langmuir : the ACS journal of surfaces and colloids.

[26]  Hyeongjin Lee,et al.  A New Approach for Fabricating Collagen/ECM‐Based Bioinks Using Preosteoblasts and Human Adipose Stem Cells , 2015, Advanced healthcare materials.

[27]  Diego Velasco,et al.  3D bioprinting of functional human skin: production and in vivo analysis , 2016, Biofabrication.

[28]  G. Tovar,et al.  Extrusion-Based 3D Printing of Poly(ethylene glycol) Diacrylate Hydrogels Containing Positively and Negatively Charged Groups , 2018, Gels.

[29]  Yong Huang,et al.  Alginate gelation-induced cell death during laser-assisted cell printing , 2014, Biofabrication.

[30]  Wim E Hennink,et al.  25th Anniversary Article: Engineering Hydrogels for Biofabrication , 2013, Advanced materials.

[31]  Jukka Rantanen,et al.  Anti‐tuberculosis drug combination for controlled oral delivery using 3D printed compartmental dosage forms: From drug product design to in vivo testing , 2017, Journal of controlled release : official journal of the Controlled Release Society.

[32]  Jae Young Lee,et al.  Cell-laden 3D bioprinting hydrogel matrix depending on different compositions for soft tissue engineering: Characterization and evaluation. , 2017, Materials science & engineering. C, Materials for biological applications.

[33]  Aizheng Chen,et al.  Characterization and Preliminary Biological Evaluation of 3D-Printed Porous Scaffolds for Engineering Bone Tissues , 2018, Materials.

[34]  Yogendra Pratap Singh,et al.  3D Bioprinting using Cross-Linker Free Silk-Gelatin Bioink for Cartilage Tissue Engineering. , 2019, ACS applied materials & interfaces.

[35]  T. Scheibel,et al.  Biofabrication of cell-loaded 3D spider silk constructs. , 2015, Angewandte Chemie.

[36]  D. D’Lima,et al.  Direct human cartilage repair using three-dimensional bioprinting technology. , 2012, Tissue engineering. Part A.

[37]  Tim B. F. Woodfield,et al.  5.14 Biofabrication in Tissue Engineering , 2017 .

[38]  Anthony Atala,et al.  3D bioprinting of tissues and organs , 2014, Nature Biotechnology.

[39]  Junhee Lee,et al.  Three-dimensional bio-printing equipment technologies for tissue engineering and regenerative medicine , 2016, Tissue Engineering and Regenerative Medicine.

[40]  Katia Bertoldi,et al.  Mathematically defined tissue engineering scaffold architectures prepared by stereolithography. , 2010, Biomaterials.

[41]  Elise M. Stewart,et al.  3D printing of layered brain-like structures using peptide modified gellan gum substrates. , 2015, Biomaterials.

[42]  Malcolm Xing,et al.  3D bioprinting for biomedical devices and tissue engineering: A review of recent trends and advances , 2018, Bioactive materials.

[43]  S. Takka,et al.  IMPROVING THE DISSOLUTION OF A WATER-INSOLUBLE ORPHAN DRUG THROUGH A FUSED DEPOSITION MODELLING 3-DIMENSIONAL PRINTING TECHNOLOGY APPROACH. , 2020, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[44]  Niklas Sandler,et al.  Inkjet printing of antiviral PCL nanoparticles and anticancer cyclodextrin inclusion complexes on bioadhesive film for cervical administration. , 2017, International journal of pharmaceutics.

[45]  S. Tasoglu,et al.  3D printing for drug manufacturing: A perspective on the future of pharmaceuticals , 2017, International journal of bioprinting.

[46]  Ira Bhatnagar,et al.  Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering , 2014, Marine drugs.

[47]  Deok‐Ho Kim,et al.  Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink , 2014, Nature Communications.

[48]  Young-Sam Cho,et al.  Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration , 2018, Tissue Engineering and Regenerative Medicine.

[49]  Anthony Atala,et al.  A hydrogel bioink toolkit for mimicking native tissue biochemical and mechanical properties in bioprinted tissue constructs. , 2015, Acta biomaterialia.

[50]  Jiawei Wang,et al.  Novel chitosan/collagen scaffold containing transforming growth factor-beta1 DNA for periodontal tissue engineering. , 2006, Biochemical and biophysical research communications.

[51]  M. Khan,et al.  A new chapter in pharmaceutical manufacturing: 3D‐printed drug products☆, ☆☆ , 2017, Advanced drug delivery reviews.

[52]  K. Satish Prakash,et al.  Additive Manufacturing Techniques in Manufacturing -An Overview , 2018 .

[53]  Wen-Ching Chang,et al.  3D Printing of Cytocompatible Water-Based Light-Cured Polyurethane with Hyaluronic Acid for Cartilage Tissue Engineering Applications , 2017, Materials.

[54]  Alan Faulkner-Jones,et al.  Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D , 2015, Biofabrication.

[55]  Carlos G. Helguero,et al.  Trabecular Scaffolds’ Mechanical Properties of Bone Reconstruction Using Biomimetic Implants , 2017 .

[56]  L. Niklason,et al.  Scaffold-free vascular tissue engineering using bioprinting. , 2009, Biomaterials.

[57]  L. Lamberti,et al.  3D printed biodegradable composites: An insight into mechanical properties of PLA/chitosan scaffold , 2020 .

[58]  Jianhua Sun,et al.  A programmed release multi-drug implant fabricated by three-dimensional printing technology for bone tuberculosis therapy , 2009, Biomedical materials.

[59]  F. Guillemot,et al.  Laser-assisted bioprinting for creating on-demand patterns of human osteoprogenitor cells and nano-hydroxyapatite , 2011, Biofabrication.

[60]  S. Soker,et al.  A tunable hydrogel system for long-term release of cell-secreted cytokines and bioprinted in situ wound cell delivery. , 2017, Journal of biomedical materials research. Part B, Applied biomaterials.

[61]  J. Connelly,et al.  Dynamic Compression Regulates the Expression and Synthesis of Chondrocyte‐Specific Matrix Molecules in Bone Marrow Stromal Cells , 2007, Stem cells.

[62]  F. Guillemot,et al.  Laser assisted bioprinting of engineered tissue with high cell density and microscale organization. , 2010, Biomaterials.

[63]  B. Duan,et al.  3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. , 2013, Journal of biomedical materials research. Part A.

[64]  P. Petrini,et al.  Advances in biomedical applications of pectin gels. , 2012, International journal of biological macromolecules.

[65]  D. Kelly,et al.  3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering , 2016, Advanced healthcare materials.

[66]  W. Koh,et al.  Mesenchymal stem cell 3D encapsulation technologies for biomimetic microenvironment in tissue regeneration , 2019, Stem Cell Research & Therapy.

[67]  Nan Ma,et al.  Laser printing of skin cells and human stem cells. , 2010, Tissue engineering. Part C, Methods.

[68]  Jeffrey L Goldberg,et al.  Control of Retinal Ganglion Cell Positioning and Neurite Growth: Combining 3D Printing with Radial Electrospun Scaffolds. , 2016, Tissue engineering. Part A.

[69]  Ying Mei,et al.  Engineering alginate as bioink for bioprinting. , 2014, Acta biomaterialia.

[70]  Seng Han Lim,et al.  3D printed drug delivery and testing systems — a passing fad or the future? , 2018, Advanced drug delivery reviews.

[71]  Vilmos Vécsei,et al.  The influence of scaffold architecture on chondrocyte distribution and behavior in matrix-associated chondrocyte transplantation grafts. , 2011, Biomaterials.

[72]  Xinggang Yang,et al.  Preparation and investigation of novel gastro-floating tablets with 3D extrusion-based printing. , 2018, International journal of pharmaceutics.

[73]  W. Hennink,et al.  Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing. , 2007, Tissue engineering.

[74]  Brian Derby,et al.  Printing and Prototyping of Tissues and Scaffolds , 2012, Science.

[75]  Robert Langer,et al.  Principles of tissue engineering , 2014 .

[76]  Bin Zhang,et al.  3D Bioprinting: A Novel Avenue for Manufacturing Tissues and Organs , 2019, Engineering.

[77]  Anthony Atala,et al.  DROP-ON-DEMAND INKJET BIOPRINTING: A PRIMER ∗ , 2011 .

[78]  Chan Hee Park,et al.  3D bioprinting for active drug delivery , 2019, Biomimetic Nanoengineered Materials for Advanced Drug Delivery.

[79]  Pankaj Karande,et al.  Design and fabrication of human skin by three-dimensional bioprinting. , 2014, Tissue engineering. Part C, Methods.

[80]  Jonathan Goole,et al.  3D printing in pharmaceutics: A new tool for designing customized drug delivery systems. , 2016, International journal of pharmaceutics.

[81]  David E. Pegg Principles of Tissue Engineering , 1999 .

[82]  J A Barron,et al.  Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns , 2004, Biomedical microdevices.

[83]  Hyeongjin Lee,et al.  Mechanically reinforced cell-laden scaffolds formed using alginate-based bioink printed onto the surface of a PCL/alginate mesh structure for regeneration of hard tissue. , 2016, Journal of colloid and interface science.

[84]  Joseph Suhan,et al.  Bioprinting of Growth Factors onto Aligned Sub-micron Fibrous Scaffolds for Simultaneous Control of Cell Differentiation and Alignment , 2022 .

[85]  Xiaodong Cao,et al.  Preparation and Properties of 3D Printed Alginate–Chitosan Polyion Complex Hydrogels for Tissue Engineering , 2018, Polymers.

[86]  P. Kluger,et al.  Advanced gelatin-based vascularization bioinks for extrusion-based bioprinting of vascularized bone equivalents , 2020, Scientific Reports.

[87]  S. Bhatia,et al.  3D Printing and Bio-Based Materials in Global Health: An Interventional Approach to the Global Burden of Surgical Disease in Low-and Middle-Income Countries , 2017 .

[88]  Elliot S. Bishop,et al.  3-D bioprinting technologies in tissue engineering and regenerative medicine: Current and future trends , 2017, Genes & diseases.

[89]  Ibrahim T. Ozbolat,et al.  3D bioprinting for drug discovery and development in pharmaceutics. , 2017, Acta biomaterialia.

[90]  Anthony Atala,et al.  A 3D bioprinted complex structure for engineering the muscle–tendon unit , 2015, Biofabrication.

[91]  Charles C. Persinger,et al.  How to improve R&D productivity: the pharmaceutical industry's grand challenge , 2010, Nature Reviews Drug Discovery.

[92]  Qian Yan,et al.  A Review of 3D Printing Technology for Medical Applications , 2018, Engineering.

[93]  Heng Wang,et al.  3D Bioprinting of Self‐Standing Silk‐Based Bioink , 2018, Advanced healthcare materials.

[94]  R. M. Owen,et al.  An analysis of the attrition of drug candidates from four major pharmaceutical companies , 2015, Nature Reviews Drug Discovery.

[95]  Fei Gao,et al.  High-strength hydrogel-based bioinks , 2019, Materials Chemistry Frontiers.

[96]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[97]  Binil Starly,et al.  3D-Bioprinting of Polylactic Acid (PLA) Nanofiber-Alginate Hydrogel Bioink Containing Human Adipose-Derived Stem Cells. , 2016, ACS biomaterials science & engineering.

[98]  Manasi Vaidya,et al.  Startups tout commercially 3D-printed tissue for drug screening , 2015, Nature Medicine.

[99]  Josep A Planell,et al.  Biofabrication of tissue constructs by 3D bioprinting of cell-laden microcarriers , 2014, Biofabrication.

[100]  Peng Pei,et al.  Three dimensional printing of calcium sulfate and mesoporous bioactive glass scaffolds for improving bone regeneration in vitro and in vivo , 2017, Scientific Reports.

[101]  Hod Lipson,et al.  Increased mixing improves hydrogel homogeneity and quality of three-dimensional printed constructs. , 2011, Tissue engineering. Part C, Methods.

[102]  F. Acartürk,et al.  An Effective Technology for the Development of Immediate Release Solid Dosage Forms Containing Low-Dose Drug: Fused Deposition Modeling 3D Printing , 2019, Pharmaceutical Research.

[103]  Jesse K. Placone,et al.  Extrusion-based 3D printing of poly(propylene fumarate) scaffolds with hydroxyapatite gradients , 2017, Journal of biomaterials science. Polymer edition.

[104]  Michael C. McAlpine,et al.  3D Printed Bionic Ears , 2013, Nano letters.

[105]  Liang Ma,et al.  3D bioprinting: an emerging technology full of opportunities and challenges , 2018, Bio-Design and Manufacturing.

[106]  Niklas Sandler,et al.  Mechanical characterization and ex vivo evaluation of anticancer and antiviral drug printed bioadhesive film for the treatment of cervical cancer , 2019, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[107]  Fan Liu,et al.  Natural Polymers for Organ 3D Bioprinting , 2018, Polymers.

[108]  L. Grover,et al.  Cell encapsulation using biopolymer gels for regenerative medicine , 2010, Biotechnology Letters.

[109]  J. Ringe,et al.  Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation , 2009, Arthritis research & therapy.

[110]  D. Mills,et al.  Bioinks and bioprinting: A focused review , 2020, Bioprinting.

[111]  Kang Zhang,et al.  3D printing of functional biomaterials for tissue engineering. , 2016, Current opinion in biotechnology.

[112]  R. Samanipour,et al.  A simple and high-resolution stereolithography-based 3D bioprinting system using visible light crosslinkable bioinks , 2015, Biofabrication.

[113]  Udayabhanu M. Jammalamadaka,et al.  Novel Biomaterials Used in Medical 3D Printing Techniques , 2018, Journal of functional biomaterials.

[114]  Rui L. Reis,et al.  Chondrogenic potential of injectable κ‐carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue‐engineering applications , 2015, Journal of tissue engineering and regenerative medicine.

[115]  Marcy Zenobi-Wong,et al.  Nanostructured Pluronic hydrogels as bioinks for 3D bioprinting , 2015, Biofabrication.

[116]  Haopeng Ma,et al.  3D printing of HA / PCL composite tissue engineering scaffolds , 2019, Advanced Industrial and Engineering Polymer Research.

[117]  Marcia Simon,et al.  Hydrogels for Regenerative Medicine , 2016 .

[118]  Anthony Atala,et al.  Evaluation of hydrogels for bio-printing applications. , 2013, Journal of biomedical materials research. Part A.

[119]  J Malda,et al.  Design criteria for a printed tissue engineering construct: a mathematical homogenization approach. , 2009, Journal of theoretical biology.

[120]  Thomas H. Jovic,et al.  Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications , 2019, Front. Mech. Eng..

[121]  Jane Wang,et al.  Study of Physical and Degradation Properties of 3D-Printed Biodegradable, Photocurable Copolymers, PGSA-co-PEGDA and PGSA-co-PCLDA , 2018, Polymers.

[122]  Niklas Sandler,et al.  Hyperspectral imaging in quality control of inkjet printed personalised dosage forms. , 2015, International journal of pharmaceutics.

[123]  D. Kenkel,et al.  Bioprinting Complex Cartilaginous Structures with Clinically Compliant Biomaterials , 2015 .

[124]  Anthony Atala,et al.  Tissue engineering of human bladder. , 2011, British medical bulletin.

[125]  Analava Mitra,et al.  Top-Down and Bottom-Up Approaches in 3D Printing Technologies for Drug Delivery Challenges. , 2015, Critical reviews in therapeutic drug carrier systems.

[126]  Jun Liu,et al.  Current advances and future perspectives of 3D printing natural-derived biopolymers. , 2019, Carbohydrate polymers.

[127]  Wouter J A Dhert,et al.  Prolonged presence of VEGF promotes vascularization in 3D bioprinted scaffolds with defined architecture. , 2014, Journal of controlled release : official journal of the Controlled Release Society.

[128]  Ursula Graf-Hausner,et al.  Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells , 2016, Journal of laboratory automation.

[129]  Niklas Sandler,et al.  Inkjet printing of drug substances and use of porous substrates-towards individualized dosing. , 2011, Journal of pharmaceutical sciences.

[130]  F. Lin,et al.  Fabrication of viable tissue-engineered constructs with 3D cell-assembly technique. , 2005, Biomaterials.

[131]  Pamela Robles Martinez,et al.  Fabrication of drug-loaded hydrogels with stereolithographic 3D printing. , 2017, International journal of pharmaceutics.

[132]  L. Mongeau,et al.  Characterization of a hierarchical network of hyaluronic acid/gelatin composite for use as a smart injectable biomaterial. , 2012, Macromolecular bioscience.

[133]  Su A Park,et al.  Novel 3D printed alginate–BFP1 hybrid scaffolds for enhanced bone regeneration , 2017 .

[134]  Udayabhanu M. Jammalamadaka,et al.  Medication eluting devices for the field of OBGYN (MEDOBGYN): 3D printed biodegradable hormone eluting constructs, a proof of concept study , 2017, PloS one.

[135]  G. Daley,et al.  The promise of induced pluripotent stem cells in research and therapy , 2012, Nature.

[136]  James J. Yoo,et al.  Bioprinting technology and its applications. , 2014, European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery.

[137]  W. Richter,et al.  Mesenchymal stem cell differentiation in an experimental cartilage defect: restriction of hypertrophy to bone-close neocartilage. , 2009, Stem cells and development.

[138]  Sawyer B. Fuller,et al.  A fast flexible ink-jet printing method for patterning dissociated neurons in culture , 2004, Journal of Neuroscience Methods.