Bio-effects of Nano-TiO2 on DNA and cellular ultrastructure with different polymorph and size

Abstract In this paper, we investigated the bio-effect of three kinds of nano-TiO2 on the aspects of DNA strand breaking activity, generation of 8-hydroxy-2-deoxyguanosine (8-OHdG), cytotoxicity and cell morphology. Different extents of strand scission and the formation of 8-OHdG in DNA were obtained after treated with different types of nano-TiO2 (10 – 20 nm anatase, 50 – 60 nm anatase and 50 – 60 nm rutile). Furthermore, MTT assay and TEM detection showed the toxic effects on CHO cells with a toxic order as 10 – 20 nm anatase > 50 – 60 nm anatase > 50 – 60 nm rutile, which was consistent with that of 8-OHdG generation and DNA strand breaking activities. It was interesting that three kind of nano TiO2 all had much higher toxic effect on tumor cells than on normal cells, and nano-TiO2 was observed in the cytoplasm of CHO cells. These results indicated that the toxic effect of nano-TiO2 relates to the size and type of particles, in addition, their potential application for cancer treatment was discussed.

[1]  Yuan Chunwei,et al.  The Study of the Photokilling Effect and Mechanism of Ultrafine TiO_2 Particles on U937 Cells , 1997 .

[2]  Xiaoli Zhu,et al.  Hemoglobin-based hydrogen peroxide biosensor tuned by the photovoltaic effect of nano titanium dioxide. , 2005, Analytical chemistry.

[3]  M. Miki-Yoshida,et al.  TEM evidence of ultrastructural alteration on Pseudomonas aeruginosa by photocatalytic TiO2 thin films. , 2003, Journal of photochemistry and photobiology. B, Biology.

[4]  David M. Brown,et al.  Size-dependent proinflammatory effects of ultrafine polystyrene particles: a role for surface area and oxidative stress in the enhanced activity of ultrafines. , 2001, Toxicology and applied pharmacology.

[5]  Robert Gelein,et al.  Role of the alveolar macrophage in lung injury: studies with ultrafine particles. , 1992 .

[6]  H. Utsumi,et al.  Quantitative determination of OH radical generation and its cytotoxicity induced by TiO(2)-UVA treatment. , 2002, Toxicology in vitro : an international journal published in association with BIBRA.

[7]  Jun-Jie Yin,et al.  Oxidative damage to nucleic acids photosensitized by titanium dioxide. , 1997, Free radical biology & medicine.

[8]  Y. Aizawa,et al.  DIFFERENCES IN THE EFFECTS OF FIBROUS AND PARTICULATE TITANIUM DIOXIDE ON ALVEOLAR MACROPHAGES OF FISCHER 344 RATS , 2002, Journal of toxicology and environmental health. Part A.

[9]  Ai-Ping Zhang,et al.  Photocatalytic killing effect of TiO2 nanoparticles on Ls-174-t human colon carcinoma cells. , 2004, World journal of gastroenterology.

[10]  F. Seiler,et al.  Investigations on the inflammatory and genotoxic lung effects of two types of titanium dioxide: untreated and surface treated. , 2003, Toxicology and applied pharmacology.

[11]  G. Oberdörster,et al.  Pulmonary effects of inhaled ultrafine particles , 2000, International archives of occupational and environmental health.

[12]  J. Gearhart,et al.  In vitro toxicity of nanoparticles in BRL 3A rat liver cells. , 2005, Toxicology in vitro : an international journal published in association with BIBRA.

[13]  J. West,et al.  Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. , 2006, Toxicological sciences : an official journal of the Society of Toxicology.

[14]  Liping Tang,et al.  Surface chemistry influences cancer killing effect of TiO2 nanoparticles. , 2008, Nanomedicine : nanotechnology, biology, and medicine.

[15]  Robert A Hoke,et al.  Development of a base set of toxicity tests using ultrafine TiO2 particles as a component of nanoparticle risk management. , 2007, Toxicology letters.

[16]  Zhongdang Xiao,et al.  Photoexcited TiO2 nanoparticles through •OH-radicals induced malignant cells to necrosis , 1998 .