A Modified Blood Stain, Useful in the Diagnosis and Evaluation of Hematologic Elements

AbstractA modified blood stain has been developed for the demonstration and differentiation of cell types in peripheral blood or marrow smears, parasites in blood, and tissue imprint preparations. It is available as a powder extracted from known quantities of dyes of unpolychromed methylene blue, azure II and eosin Y. The powder keeps indefinitely without any appreciable loss of potency, and it is dissolved in 100% methanol or in equal parts of glycerol and methanol. The method of staining is simple and rapid, and the tinctorial patterns closely resemble those of the conventional blood stains, such as Giemsa, Wright, and Leishman. It is specific, stable, reproducible, and as the stain solution ages the staining quality improves.