Graphical methods for three-dimensional rotation of complex axonal arborizations

Methods are described for 3-dimensional visualization and spatial manipulation of complex axonal arborizations. These methods allow 2-dimensional camera lucida drawings of neuron processes passing through serial sections to be redrawn with an altered viewing perspective without need for a computer. Two methods are described: a method for 90 degrees rotation, and a method for approximating smaller angle rotations. The method for small angle rotation was developed to visualize the spatial relationships between cortical laminae (curved as well as planar) and axon branching patterns.

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