Integrated Optofluidic Chip for Low-Volume Fluid Viscosity Measurement

In the present work, an integrated optofluidic chip for fluid viscosity measurements in the range from 1 mPa·s to 100 mPa·s is proposed. The device allows the use of small sample volumes (<1 µL) and the measurement of viscosity as a function of temperature. Thanks to the precise control of the force exerted on dielectric spheres by optical beams, the viscosity of fluids is assessed by comparing the experimentally observed movement of dielectric beads produced by the optical forces with that expected by numerical calculations. The chip and the developed technique are validated by analyzing several fluids, such as Milli-Q water, ethanol and water–glycerol mixtures. The results show a good agreement between the experimental values and those reported in the literature. The extremely reduced volume of the sample required and the high flexibility of this technique make it a good candidate for measuring a wide range of viscosity values as well as for the analysis of nonlinear viscosity in complex fluids.

[1]  F. Bragheri,et al.  Optofluidic integrated cell sorter fabricated by femtosecond lasers. , 2012, Lab on a chip.

[2]  Paolo Minzioni,et al.  A Comprehensive Review of Optical Stretcher for Cell Mechanical Characterization at Single-Cell Level , 2016, Micromachines.

[3]  R. Robertson-Anderson,et al.  Nonlinear microrheology reveals entanglement-driven molecular-level viscoelasticity of concentrated DNA. , 2014, Physical review letters.

[4]  A. Ashkin Acceleration and trapping of particles by radiation pressure , 1970 .

[5]  Miles J Padgett,et al.  Measuring storage and loss moduli using optical tweezers: broadband microrheology. , 2009, Physical review. E, Statistical, nonlinear, and soft matter physics.

[6]  Mason,et al.  Linear viscoelasticity of colloidal hard sphere suspensions near the glass transition. , 1995, Physical review letters.

[7]  F. Bragheri,et al.  A Novel Approach to Fiber-Optic Tweezers: Numerical Analysis of the Trapping Efficiency , 2008, IEEE Journal of Selected Topics in Quantum Electronics.

[8]  Ruiping Yang,et al.  Optical force exerted on a Rayleigh particle by a vector arbitrary-order Bessel beam , 2016 .

[9]  K. Svoboda,et al.  Biological applications of optical forces. , 1994, Annual review of biophysics and biomolecular structure.

[10]  N. Cheng Formula for the Viscosity of a Glycerol−Water Mixture , 2008 .

[11]  Ilaria Cristiani,et al.  All-silica microfluidic optical stretcher with acoustophoretic prefocusing , 2015 .

[12]  Jay X. Tang,et al.  Correlated fluctuations of microparticles in viscoelastic solutions: quantitative measurement of material properties by microrheology in the presence of optical traps. , 2006, Physical review. E, Statistical, nonlinear, and soft matter physics.

[13]  Arthur Ashkin,et al.  Trapping of Atoms by Resonance Radiation Pressure , 1978 .

[14]  Jonathan Leach,et al.  Multipoint viscosity measurements in microfluidic channels using optical tweezers. , 2009, Lab on a chip.

[15]  G. W. C. Kaye,et al.  Tables of Physical and Chemical Constants , 2018 .

[16]  D A Weitz,et al.  Microrheology of entangled F-actin solutions. , 2003, Physical review letters.

[17]  Kort Travis,et al.  Fluorescence ratio thermometry in a microfluidic dual-beam laser trap. , 2007, Optics express.

[18]  Ilaria Cristiani,et al.  Experimental study of the optical forces exerted by a Gaussian beam within the Rayleigh range , 2011 .

[19]  Qiyin Fang,et al.  Optofluidic Device Based Microflow Cytometers for Particle/Cell Detection: A Review , 2016, Micromachines.

[20]  Pier Luca Maffettone,et al.  Microrheology with Optical Tweezers: Measuring the relative viscosity of solutions ‘at a glance' , 2015, Scientific Reports.

[21]  R. Osellame,et al.  Femtosecond laser fabricated monolithic chip for optical trapping and stretching of single cells. , 2010, Optics express.

[22]  I Cristiani,et al.  An integrated optofluidic device for single-cell sorting driven by mechanical properties. , 2015, Lab on a chip.

[23]  Todd M. Squires,et al.  Fluid Mechanics of Microrheology , 2010 .

[24]  A. Ashkin,et al.  Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime. , 1992, Biophysical journal.

[25]  R. Osellame,et al.  Optofluidic chip for single cell trapping and stretching fabricated by a femtosecond laser , 2010, Journal of biophotonics.

[26]  Rafael J. Taboryski,et al.  Fiber-Based, Injection-Molded Optofluidic Systems: Improvements in Assembly and Applications , 2015, Micromachines.