OsNAC52, a rice NAC transcription factor, potentially responds to ABA and confers drought tolerance in transgenic plants

A novel NAC (NAM, ATAF1/2, CUC2) homologous gene, OsNAC52, was isolated from Oryza sativa L using RACE (rapid amplification of cDNA ends). Analysis of the amino acid sequence of OsNAC52 revealed a highly conserved NAC domain and a potential nuclear localization sequence in its N-terminus, and the transcriptional activation motif in the C-terminal region. Transgenic plants over-expressing OsNAC52 were highly sensitive to ABA (Abscisic acid), and the growth of the 35S-OsNAC52 transgenic seedlings was significantly more restrained by ABA treatment than those of the wild-type seedlings. Furthermore, over-expression of OsNAC52 activated the expression of downstream genes in transgenic Arabidopsis, resulting in enhanced tolerance to drought stresses but not growth retardation. The results from this study indicate that this novel rice OsNAC52 gene functions as an important transcriptional activator in ABA-inducible gene expression and may be useful in improving plant tolerance to abiotic stress.

[1]  C. Jansson,et al.  A Novel WRKY Transcription Factor, SUSIBA2, Participates in Sugar Signaling in Barley by Binding to the Sugar-Responsive Elements of the iso1 Promoter Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.0145 , 2003, The Plant Cell Online.

[2]  J. Randles,et al.  A NAC Domain Protein Interacts with Tomato leaf curl virus Replication Accessory Protein and Enhances Viral Replication , 2005, The Plant Cell Online.

[3]  D. Van Der Straeten,et al.  The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis. , 2005, The Plant journal : for cell and molecular biology.

[4]  Kazuo Shinozaki,et al.  Isolation and Functional Analysis of Arabidopsis Stress-Inducible NAC Transcription Factors That Bind to a Drought-Responsive cis-Element in the early responsive to dehydration stress 1 Promoterw⃞ , 2004, The Plant Cell Online.

[5]  J.-S. Zhang,et al.  An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress , 2003, Theoretical and Applied Genetics.

[6]  H Fujisawa,et al.  Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. , 1997, The Plant cell.

[7]  Rossana Henriques,et al.  Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method , 2006, Nature Protocols.

[8]  J. Mol,et al.  The No Apical Meristem Gene of Petunia Is Required for Pattern Formation in Embryos and Flowers and Is Expressed at Meristem and Primordia Boundaries , 1996, Cell.

[9]  D. McCarty,et al.  Genetic control of abscisic acid biosynthesis in maize. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[10]  K. Shinozaki,et al.  OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. , 2003, The Plant journal : for cell and molecular biology.

[11]  Kazuo Shinozaki,et al.  A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. , 2004, The Plant journal : for cell and molecular biology.

[12]  S. J. Gilmour,et al.  Molecular Cloning and Expression of cor (Cold-Regulated) Genes in Arabidopsis thaliana. , 1990, Plant physiology.

[13]  K. Shinozaki,et al.  Regulatory network of gene expression in the drought and cold stress responses. , 2003, Current opinion in plant biology.

[14]  Xing Wang Deng,et al.  Genome-Wide ORFeome Cloning and Analysis of Arabidopsis Transcription Factor Genes1[w] , 2004, Plant Physiology.

[15]  Addie Nina Olsen,et al.  NAC transcription factors: structurally distinct, functionally diverse. , 2005, Trends in plant science.

[16]  Tieyan Liu,et al.  Transcription Factors in Rice: A Genome-wide Comparative Analysis between Monocots and Eudicots , 2005, Plant Molecular Biology.

[17]  E. Meyerowitz,et al.  A Homolog of NO APICAL MERISTEM Is an Immediate Target of the Floral Homeotic Genes APETALA3/PISTILLATA , 1998, Cell.

[18]  K. Shinozaki,et al.  Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice. , 2007, The Plant journal : for cell and molecular biology.

[19]  D. Klessig,et al.  MAPK cascades in plant defense signaling. , 2001, Trends in plant science.

[20]  A. Xiong,et al.  Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza Sativa L. , 2007, Molecular Breeding.

[21]  Shoshi Kikuchi,et al.  Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. , 2003, DNA research : an international journal for rapid publication of reports on genes and genomes.

[22]  K. Shinozaki,et al.  Molecular Cloning and Characterization of 9 cDNAs for Genes That Are Responsive to Desiccation in Arabidopsis thaliana: SequenceAnalysis of One cDNA Clone That Encodes a Putative Transmembrane Channel Protein , 1992 .

[23]  A. Webb,et al.  ABI1 Protein Phosphatase 2C Is a Negative Regulator of Abscisic Acid Signaling , 1999, Plant Cell.

[24]  Jian-Kang Zhu,et al.  Salt and drought stress signal transduction in plants. , 2002, Annual review of plant biology.

[25]  K. Shinozaki,et al.  Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. , 2005, Trends in plant science.

[26]  M. K. Jensen,et al.  The HvNAC6 transcription factor: a positive regulator of penetration resistance in barley and Arabidopsis , 2007, Plant Molecular Biology.

[27]  Jan-Peter Nap,et al.  The release of genetically modified crops into the environment. Part I. Overview of current status and regulations. , 2003, The Plant journal : for cell and molecular biology.

[28]  Hur-Song Chang,et al.  Expression Profile Matrix of Arabidopsis Transcription Factor Genes Suggests Their Putative Functions in Response to Environmental Stresses , 2002, The Plant Cell Online.

[29]  M. Thomashow.  Role of cold-responsive genes in plant freezing tolerance. , 1998, Plant physiology.

[30]  P. Turner,et al.  A comparison of eukaryotic viral 5'-leader sequences as enhancers of mRNA expression in vivo. , 1987, Nucleic acids research.